Related Experiment Video

Updated: Feb 17, 2026

T and B Cell Receptor Immune Repertoire Analysis using Next-generation Sequencing
08:59

T and B Cell Receptor Immune Repertoire Analysis using Next-generation Sequencing

Published on: January 12, 2021

8.9K

Gene Profiling and T Cell Receptor Sequencing from Antigen-Specific CD4 T Cells

Marie Holt1, Anne Costanzo1, Louis Gioia2

  • 1Department of Immunology and Microbiology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA, 92037, USA.

Methods in Molecular Biology (Clifton, N.J.)
|December 11, 2017
PubMed

Insights

Single-cell analysis can now identify rare T cells in blood for diagnosing autoimmune diseases and monitoring treatments. This approach overcomes limitations of bulk analysis for better patient insights.

Area of Science:

  • Immunology
  • Cell Biology
  • Medical Diagnostics

Background:

  • Bulk analysis of circulating blood cells offers limited insights due to the scarcity of pathogenic T cells.
  • Autoimmune diseases require precise diagnostic and monitoring tools for effective management.

Purpose of the Study:

  • To develop advanced single-cell analysis methods for detecting and characterizing rare circulating CD4 T cells.
  • To enable better diagnosis and treatment monitoring for autoimmune conditions.

Main Methods:

  • Implementation of novel single-cell analysis techniques.
  • Focus on identifying and characterizing specific T cell populations (CD4 T cells).

Main Results:

  • Successful development of single-cell analysis approaches.
  • Capability to identify and characterize rare circulating CD4 T cells.

Conclusions:

  • Single-cell analysis provides a powerful tool to overcome the limitations of bulk analysis.
  • These methods hold promise for improving the diagnosis and monitoring of autoimmune diseases.

Keywords:
AutoimmunityCD4 T cellsMicrofluidicsPeripheral bloodSingle-cell analysisTCR sequences

More Related Videos

Generation of Human Alloantigen-specific T Cells from Peripheral Blood09:47

Generation of Human Alloantigen-specific T Cells from Peripheral Blood

Published on: November 21, 2014

13.6K
Quantification of Proliferating Human Antigen-specific CD4+ T Cells using Carboxyfluorescein Succinimidyl Ester07:00

Quantification of Proliferating Human Antigen-specific CD4+ T Cells using Carboxyfluorescein Succinimidyl Ester

Published on: June 4, 2019

9.2K

Related Concept Videos

T Cell Activation and Clonal Selection01:22

T Cell Activation and Clonal Selection

T cells are integral to our adaptive immune system, recognizing and effectively responding to foreign antigens. T cell activation and clonal selection are pivotal in orchestrating this immune response. This article elucidates these mechanisms, detailing the roles of cluster of differentiation (CD) markers, major histocompatibility complex (MHC) molecules, costimulatory signals, and the process of clonal selection.
Naive T cells that have not yet encountered an antigen express two primary CD...
16.4K
Diversity of Antigen Receptors01:28

Diversity of Antigen Receptors

Antigen receptors are essential components of the immune system crucial in defending the body against foreign invaders. These receptors are present on the surface of B and T cells, enabling them to recognize antigens and mount an appropriate immune response.
Before encountering any antigen, lymphocytes express these receptors. On B cells, the antigen receptor is a membrane-bound antibody molecule called BCR; on T cells, it is a T cell receptor or TCR. B and T cell receptors are composed of two...
1.7K