Related Experiment Video

Updated: Feb 17, 2026

Native Polyacrylamide Gel Electrophoresis Immunoblot Analysis of Endogenous IRF5 Dimerization
08:57

Native Polyacrylamide Gel Electrophoresis Immunoblot Analysis of Endogenous IRF5 Dimerization

Published on: October 6, 2019

10.8K

IRF8-dependent molecular complexes control the Th9 transcriptional program

Etienne Humblin1,2, Marion Thibaudin1,2, Fanny Chalmin2

  • 1Univ. Bourgogne Franche-Comté, F-21000, Dijon, France.

Nature Communications
|December 14, 2017
PubMed

Insights

Interferon regulatory factor 8 (IRF8) is crucial for Th9 cell differentiation and function. This study reveals IRF8

Area of Science:

  • Immunology
  • Molecular Biology
  • Cell Biology

Background:

  • Interferon regulatory factors (IRFs) are key regulators of immune responses.
  • IRF4's role in CD4+ T cells is well-studied, but IRF8's function remains less understood.

Purpose of the Study:

  • To investigate the role of IRF8 in Th9 cell differentiation and function.
  • To elucidate the molecular mechanisms by which IRF8 regulates Th9 cell development.

Main Methods:

  • In vitro differentiation of Th9 cells.
  • In vivo studies using mouse models.
  • Analysis of transcription factor complexes and gene expression (Il9, Il4).

Main Results:

  • IRF8 is essential for Th9 differentiation both in vitro and in vivo.
  • IRF8 forms a complex with IRF4, PU.1, and BATF to enhance Il9 transcription.
  • IRF8, in complex with ETV6, represses Il4 expression.
  • IRF8-dependent Th9 cells exhibit anti-tumour effects in melanoma models.

Conclusions:

  • IRF8 plays a critical role in promoting the Th9 cell program and suppressing Il4 expression.
  • IRF8 modulates Th9 cell differentiation through specific transcription factor complexes.
  • IRF8 represents a potential therapeutic target for enhancing Th9 responses in cancer therapy.

More Related Videos

A High Resolution Method to Monitor Phosphorylation-dependent Activation of IRF311:44

A High Resolution Method to Monitor Phosphorylation-dependent Activation of IRF3

Published on: January 24, 2016

12.5K
Retroviral Transduction of Helper T Cells as a Genetic Approach to Study Mechanisms Controlling their Differentiation and Function11:50

Retroviral Transduction of Helper T Cells as a Genetic Approach to Study Mechanisms Controlling their Differentiation and Function

Published on: November 4, 2016

11.6K

Related Concept Videos

Master Transcription Regulators02:23

Master Transcription Regulators

Master transcription regulators are regulatory proteins that are predominantly responsible for regulating the expression of multiple genes. Often these genes work in concert to drive a  complex process. Activation of a master transcription regulator can lead to a cascade of transcriptional activation necessary for that outcome. These regulators can directly bind to the regulatory sequences of the various genes involved, or they can indirectly regulate transcription by binding to regulatory...
7.9K
Master Transcription Regulators02:23

Master Transcription Regulators

2.8K
General Transcription Factors01:30

General Transcription Factors

Tissue-specific transcription factors contribute to diverse cellular functions in mammals. For example, the gene for beta globin, a major component of hemoglobin, is present in all cells of the body. However, it is only expressed in red blood cells because the transcription factors that can bind to the promoter sequences of the beta globin gene are only expressed in these cells. Tissue-specific transcription factors also ensure that mutations in these factors may impair only the function of...
7.2K
RNA Polymerase II Accessory Proteins02:36

RNA Polymerase II Accessory Proteins

Proteins that regulate transcription can do so either via direct contact with RNA Polymerase or through indirect interactions facilitated by adaptors, mediators, histone-modifying proteins, and nucleosome remodelers. Direct interactions to activate transcription is seen in bacteria as well as in some eukaryotic genes. In these cases, upstream activation sequences are adjacent to the promoters, and the activator proteins interact directly with the transcriptional machinery. For example, in...
11.1K
Transcription Factors02:16

Transcription Factors

Tissue-specific transcription factors contribute to diverse cellular functions in mammals. For example, the gene for beta globin, a major component of hemoglobin, is present in all cells of the body. However, it is only expressed in red blood cells because the transcription factors that can bind to the promoter sequences of the beta globin gene are only expressed in these cells. Tissue-specific transcription factors also ensure that mutations in these factors may impair only the function of...
83.0K
Co-activators and Co-repressors02:04

Co-activators and Co-repressors

Gene transcription is regulated by the synergistic action of several proteins that form a complex at a gene regulatory site. This is observed in eukaryotes, where the regulation of gene expression is a complex process. Regulatory proteins in eukaryotes can broadly be classified into two types – regulators that bind directly to specific DNA sequences and co-regulators that associate with regulatory proteins but cannot directly bind to the DNA. These co-regulators are further divided into...
8.7K