Related Experiment Video

Updated: Feb 15, 2026

Dissecting Multi-protein Signaling Complexes by Bimolecular Complementation Affinity Purification BiCAP
06:45

Dissecting Multi-protein Signaling Complexes by Bimolecular Complementation Affinity Purification BiCAP

Published on: June 15, 2018

8.0K

Dissecting Interleukin-6 Classic- and Trans-Signaling in Inflammation and Cancer

Christoph Garbers1, Stefan Rose-John2

  • 1Institute of Biochemistry, Kiel University, Kiel, Germany.

Methods in Molecular Biology (Clifton, N.J.)
|January 12, 2018
PubMed

Insights

This study differentiates Interleukin-6 (IL-6) classic signaling from IL-6 trans-signaling. Researchers developed tools to analyze the in vivo consequences of these distinct cellular IL-6 signaling pathways.

Area of Science:

  • Immunology
  • Molecular Biology
  • Cellular Signaling

Background:

  • Interleukin-6 (IL-6) is a cytokine produced by various human cells.
  • IL-6 signaling occurs through membrane-bound IL-6 receptor (IL-6R) or soluble IL-6R (sIL-6R).
  • Classic signaling involves IL-6 binding to membrane-bound IL-6R, while trans-signaling uses sIL-6R.

Purpose of the Study:

  • To develop molecular tools for distinguishing IL-6 classic signaling from IL-6 trans-signaling.
  • To investigate the in vivo consequences of cellular IL-6 signaling.

Main Methods:

  • Generation of novel molecular tools.
  • Differentiation assays for IL-6 classic and trans-signaling pathways.
  • In vivo analysis of cellular IL-6 signaling.

Main Results:

  • Successful creation of tools to differentiate between IL-6 classic and trans-signaling.
  • Enabled analysis of the distinct downstream effects of each signaling pathway in vivo.
  • Provided insights into the JAK/STAT and MAPK intracellular signaling cascades initiated by IL-6.

Conclusions:

  • The developed tools are crucial for dissecting the specific roles of IL-6 classic and trans-signaling in biological processes.
  • This research facilitates a deeper understanding of IL-6 mediated cellular responses in vivo.
  • Future studies can leverage these tools to explore therapeutic strategies targeting specific IL-6 signaling pathways.

Keywords:
CancerIL-6 trans-signalingInflammationInterleukin-6Interleukin-6 receptorSoluble gp130

More Related Videos

Induction of Ocular Surface Inflammation and Collection of Involved Tissues06:38

Induction of Ocular Surface Inflammation and Collection of Involved Tissues

Published on: August 4, 2022

2.9K
Whisker-signaled Eyeblink Classical Conditioning in Head-fixed Mice10:14

Whisker-signaled Eyeblink Classical Conditioning in Head-fixed Mice

Published on: March 30, 2016

13.4K

Related Concept Videos

Inflammation01:38

Inflammation

Overview
62.5K
mTOR Signaling and Cancer Progression03:03

mTOR Signaling and Cancer Progression

The mammalian target of rapamycin or mTOR protein was discovered in 1994 due to its direct interaction with rapamycin. The protein gets its name from a yeast homolog called TOR. The mTOR protein complex in mammalian cells plays a major role in balancing anabolic processes such as the synthesis of proteins, lipids, and nucleotides and catabolic processes, such as autophagy in response to environmental cues, such as availability of nutrients and growth factors.
The mTOR pathway or the...
4.9K
Classical Conditioning01:18

Classical Conditioning

Associative learning, a core principle in behavioral psychology, involves forming connections between events and facilitating learned responses. This concept is vividly illustrated by classical conditioning, a process extensively studied by the Russian physiologist Ivan Pavlov. Pavlov's pioneering research on dogs' digestive systems led to the discovery that behaviors can be learned through association, laying the groundwork for classical conditioning.
Ivan Pavlov observed that dogs...
2.6K
Principles of Classical Conditioning01:23

Principles of Classical Conditioning

Classical conditioning, as described by Ivan Pavlov, is a foundational concept in associative learning, where a neutral stimulus becomes capable of eliciting a conditioned response through association with an unconditioned stimulus. The process of acquisition, where this learning occurs, and the subsequent phenomena of contiguity, contingency, generalization, discrimination, extinction, and spontaneous recovery are crucial for a comprehensive understanding of classical conditioning.
During the...
2.2K
Classical Conditioning in Daily Life01:17

Classical Conditioning in Daily Life

Classical conditioning, a fundamental principle of associative learning, explains various phenomena observed in daily life, such as fear development, the placebo effect, taste aversion, and drug habituation. These applications demonstrate the profound impact of associative learning on human behavior and physiological responses.
John B. Watson and Rosalie Rayner famously demonstrated the development of fear through classical conditioning in their experiment with Little Albert. They paired the...
2.4K
Real-World Application of Classical Conditioning01:15

Real-World Application of Classical Conditioning

Classical conditioning not only includes the initial pairing of stimuli but also extends to more complex forms, such as higher-order conditioning. Higher-order conditioning involves creating associations beyond the primary conditioned stimulus, resulting in a chain of conditioned responses.
Higher-order, or second-order, conditioning occurs when a neutral stimulus becomes associated with an already established conditioned stimulus through repeated pairings. For instance, if a dog has been...
1.5K