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Isolation and Flow Cytometric Characterization of Murine Small Intestinal Lymphocytes
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Isolation and Characterization of Mouse Intrahepatic Lymphocytes by Flow Cytometry

Florian Wiede1,2, Tony Tiganis3,4

  • 1Biomedicine Discovery Institute, Department of Biochemistry and Molecular Biology, Monash University, Melbourne, VIC, Australia. Florian.Wiede@monash.edu.

Methods in Molecular Biology (Clifton, N.J.)
|January 12, 2018
PubMed

Insights

This study presents an efficient method for isolating viable mouse intrahepatic lymphocytes (IHL) from liver tissue for multicolor flow cytometry analysis. The new technique improves cell yields and viability for immune cell characterization.

Area of Science:

  • Cellular immunology
  • Immunological methods
  • Flow cytometry

Background:

  • Multicolor flow cytometry is crucial in cellular immunology for analyzing immune cell subsets.
  • Isolating immune cells from non-lymphoid tissues, like the liver, presents challenges in maintaining cell yield and viability.

Purpose of the Study:

  • To develop an efficient method for isolating viable mouse intrahepatic lymphocytes (IHL).
  • To enable multicolor flow cytometry characterization of IHL from normal and cancerous liver tissues.

Main Methods:

  • Described a novel method for isolating intrahepatic lymphocytes (IHL) from mouse liver.
  • Utilized multicolor flow cytometry for characterizing isolated IHL.

Main Results:

  • The method ensures efficient isolation of viable mouse intrahepatic lymphocytes (IHL).
  • Successfully characterized immune cell subsets from both normal and liver cancer tissues.

Conclusions:

  • The developed method enhances the isolation of viable IHL for flow cytometry.
  • This technique facilitates detailed immune cell analysis in liver immunology research.

Keywords:
Flow cytometryFluorophore-conjugated antibodiesHepatocellular carcinomaIntrahepatic lymphocyte isolationLiver tumors

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