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Immunohistochemistry of the Carotid Body

Jayasri Nanduri1, Nanduri R Prabhakar2

  • 1Institute for Integrative Physiology, Center for Systems Biology of Oxygen Sensing, Biological Sciences Division, Department of Medicine, University of Chicago, Chicago, IL, USA.

Methods in Molecular Biology (Clifton, N.J.)
|January 14, 2018
PubMed

Insights

Immunohistochemistry (IHC) is vital for studying oxygen sensing in the carotid body (CB). This chapter details IHC methods for both CB tissue and isolated CB cells, enhancing research in this area.

Area of Science:

  • Physiology
  • Cell Biology
  • Histology

Background:

  • The carotid body (CB) is a crucial oxygen-sensing organ.
  • Understanding protein distribution in the CB is essential for studying its function.
  • Immunohistochemistry (IHC) is a key technique for visualizing proteins within cells.

Purpose of the Study:

  • To provide a comprehensive guide to performing IHC on carotid body (CB) samples.
  • To detail the application of IHC for analyzing protein expression and localization in CB tissue and isolated cells.

Main Methods:

  • Detailed protocol for immunohistochemistry (IHC) on carotid body (CB) tissue sections.
  • Methodology for applying IHC to isolated CB cells.
  • Standard IHC staining and detection procedures.

Main Results:

  • Demonstration of protein localization within the CB using IHC.
  • Visualization of cellular and subcellular protein distribution.
  • Successful application of IHC for studying oxygen-sensing mechanisms.

Conclusions:

  • Immunohistochemistry is an indispensable technique for CB research.
  • The described IHC methods facilitate detailed analysis of protein expression in the CB.
  • This chapter serves as a practical resource for researchers studying CB physiology and oxygen sensing.

Keywords:
Antigen retrievalBlocking reagentsCarotid bifurcationFluorophoresPrimary and secondary antibodies

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