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Updated: Feb 15, 2026

Generation and Isolation of Cell Cycle-arrested Cells with Complex Karyotypes
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Generation and Isolation of Cell Cycle-arrested Cells with Complex Karyotypes

Published on: April 13, 2018

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Leukemic phase and CSF involvement of diffuse large B-cell lymphoma with a complex karyotype including a TP53

Jillian Sinkoff1, Horatiu Olteanu2, Jess F Peterson2

  • 1Department of Medicine Medical College of Wisconsin Milwaukee Wisconsin.

Clinical Case Reports
|January 30, 2018
PubMed

Insights

Diffuse large B-cell lymphoma can rarely present as leukemia, mimicking other lymphoid diseases. Advanced diagnostics like immunophenotyping, FISH, and karyotyping are crucial for accurate diagnosis and effective disease management.

Area of Science:

  • Hematology
  • Oncology
  • Diagnostic Pathology

Background:

  • Diffuse large B-cell lymphoma (DLBCL) is a common non-Hodgkin lymphoma.
  • DLBCL can uncommonly manifest with leukemic involvement, presenting a diagnostic challenge.
  • Leukemic presentation of DLBCL can be mistaken for other lymphoid malignancies.

Purpose of the Study:

  • To highlight the diagnostic complexities of leukemic phase DLBCL.
  • To emphasize the importance of advanced diagnostic techniques in differentiating DLBCL from other lymphoid disorders.
  • To underscore the critical role of accurate diagnosis in guiding DLBCL management.

Main Methods:

  • Review of clinical presentations of DLBCL in leukemic phase.
  • Application of immunophenotyping for cell surface marker analysis.
  • Utilization of Fluorescence In Situ Hybridization (FISH) for detecting genetic abnormalities.
  • Performance of karyotyping to assess chromosomal alterations.

Main Results:

  • Leukemic phase DLBCL presents a significant diagnostic challenge due to overlapping features with other lymphoid diseases.
  • Immunophenotyping, FISH analysis, and karyotyping are essential tools for definitive diagnosis.
  • Accurate identification of DLBCL subtype is critical for appropriate therapeutic strategies.

Conclusions:

  • Leukemic presentation of DLBCL requires a high index of suspicion.
  • A combination of advanced diagnostic methods is indispensable for accurate diagnosis.
  • Timely and precise diagnosis of leukemic DLBCL ensures optimal patient management and outcomes.

Keywords:
DLBCLNHLTP53 deletion

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