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Functional CD169 on Macrophages Mediates Interaction with Dendritic Cells for CD8+ T Cell Cross-Priming

Dieke van Dinther1, Henrike Veninga1, Salvador Iborra2

  • 1Cancer Center Amsterdam, Department of Molecular Cell Biology and Immunology, VU University Medical Center, Amsterdam, the Netherlands.

Cell Reports
|February 10, 2018
PubMed

Insights

Splenic CD169+ macrophages and CD8α+ dendritic cells collaborate to initiate CD8+ T cell responses. This interaction, crucial for immunity, relies on CD169 binding to sialic acids on dendritic cells.

Area of Science:

  • Immunology
  • Cell Biology
  • Microbiology

Background:

  • Splenic CD169+ macrophages capture blood-borne pathogens in the marginal zone.
  • CD8+ T cell responses are critical for adaptive immunity against various infections.

Purpose of the Study:

  • Investigate the requirements for CD169+ macrophage-mediated induction of CD8+ T cell responses.
  • Elucidate the role of CD169 and CD8α+ dendritic cells in antigen presentation and T cell activation.

Main Methods:

  • Antigen targeting to CD169+ macrophages.
  • Analysis of T cell priming and activation.
  • Assessment of CD169 binding capacity and functional relevance.

Main Results:

  • BATF3-dependent CD8α+ dendritic cells are essential for DNGR-1-mediated CD8+ T cell cross-priming.
  • CD169 preferentially binds to CD8α+ dendritic cells via sialic acid.
  • CD169's sialic acid-binding capacity is critical for antigen transfer and T cell activation.
  • Functional CD169 enhances CD8+ T cell responses during modified vaccinia Ankara virus infection.

Conclusions:

  • CD169+ macrophages and CD8α+ dendritic cells collaborate for effective CD8+ T cell responses.
  • CD169-sialic acid interaction facilitates antigen transfer and T cell activation, crucial for cellular immunity.

Keywords:
CD169DNGR-1SialoadhesinSiglec-1T cell responseantigencross-presentationdendritic cellmacrophagevaccinia

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