Related Experiment Video

Updated: Feb 14, 2026

Isolation of Sertoli Cells and Peritubular Cells from Rat Testes
11:11

Isolation of Sertoli Cells and Peritubular Cells from Rat Testes

Published on: February 8, 2016

23.5K

Rabbit Sertoli Cells: Immunohistochemical Profile from Neonatal to Adult Age

Valeria Grieco1, Barbara Banco2

  • 1Reparto di Anatomia Patologica, DiMeVet, Università degli Studi di Milano, Milan, Italy. valeria.grieco@unimi.it.

Methods in Molecular Biology (Clifton, N.J.)
|February 18, 2018
PubMed

Insights

Immunohistochemistry (IHC) defines rabbit Sertoli cell immunophenotypes using the avidin-biotin complex (ABC) method. This technique visualizes antigens in tissue, aiding in understanding cell development from neonatal to adult stages.

Area of Science:

  • Reproductive Biology
  • Cell Biology
  • Histology

Background:

  • Immunohistochemistry (IHC) is crucial for visualizing antigens in tissues using specific antibodies.
  • It aids in defining cell immunophenotypes during maturation and neoplastic transformation.
  • Understanding Sertoli cell development is vital in reproductive biology.

Purpose of the Study:

  • To investigate the immunophenotype of postnatal rabbit Sertoli cells.
  • To track changes in Sertoli cell characteristics from neonatal to adult stages.
  • To detail a common immunohistochemical protocol for this investigation.

Main Methods:

  • Formalin-fixed paraffin-embedded tissue specimens were used.
  • The avidin-biotin complex (ABC) method, a common IHC protocol, was employed.
  • Microscopical observation was performed to analyze antigen demonstration.

Main Results:

  • The study successfully applied IHC to rabbit Sertoli cells.
  • The avidin-biotin complex method allowed for antigen visualization.
  • Immunophenotypic changes during postnatal development were investigated.

Conclusions:

  • Immunohistochemistry is an effective tool for studying rabbit Sertoli cell development.
  • The avidin-biotin complex method is suitable for characterizing Sertoli cell immunophenotypes.
  • This research contributes to understanding Sertoli cell maturation in rabbits.

Keywords:
AntibodiesAntigensChromogenFormalin-fixed specimensSertoli cells

More Related Videos

Single Cell Transcriptional Profiling of Adult Mouse Cardiomyocytes08:23

Single Cell Transcriptional Profiling of Adult Mouse Cardiomyocytes

Published on: December 28, 2011

18.1K
Blind Endotracheal Intubation in Neonatal Rabbits04:04

Blind Endotracheal Intubation in Neonatal Rabbits

Published on: February 26, 2021

6.0K

Related Concept Videos

Adult Stem Cells01:33

Adult Stem Cells

Stem cells are undifferentiated cells that divide and produce more stem cells or progenitor cells that differentiate into mature, specialized cell types. All the cells in the body are generated from stem cells in the early embryo, but small populations of stem cells are also present in many adult tissues including the bone marrow, brain, skin, and gut. These adult stem cells typically produce the various cell types found in that tissue—to replace cells that are damaged or to continuously...
33.9K
Aging01:26

Aging

Aging is a complex biological phenomenon influenced by various processes that affect cellular and systemic functions. Several prominent theories attempt to explain its mechanisms, highlighting cellular limitations, oxidative damage, and hormonal changes as central factors in aging.
Cellular Clock Theory
The cellular clock theory posits that the human lifespan is closely tied to the finite capacity of cells to divide, a phenomenon governed by telomeres, which are protective caps at the ends of...
844
The Effect of Aging on Tissues01:19

The Effect of Aging on Tissues

Several body functions deteriorate with age. The external signs of aging are easily identifiable. For example, the skin becomes dry, less elastic, and thins out, forming wrinkles. The skin of the face begins to appear looser due to a decrease in the levels of elastic and collagen fibers in the connective tissue. Additionally, melanin production in the hair follicle decreases with age, resulting in gray hair. Moreover, the senses of sight and hearing decline, so glasses and hearing aids may...
3.7K
Ribosome Profiling02:24

Ribosome Profiling

Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
4.2K
Changes in the Appendicular Skeleton with Age01:09

Changes in the Appendicular Skeleton with Age

The upper and lower limb initially develops as a small bulge called a limb bud, which appears on the lateral side of the early embryo. The upper limb bud appears near the end of the fourth week of development, with the lower limb bud appearing shortly after.
Initially, the limb buds consist of a core of mesenchyme covered by a layer of ectoderm. The ectoderm at the end of the limb bud thickens to form a narrow crest called the apical ectodermal ridge. This ridge stimulates the underlying...
3.7K
Distinctive Features of Adult Stem Cells vs Cancer Stem Cells01:18

Distinctive Features of Adult Stem Cells vs Cancer Stem Cells

A stem cell is an unspecialized cell that can divide without limit as needed and can, under specific conditions, differentiate into specialized cells.
Adult stem cells
Adult stem cells are tissue-specific; hence, they divide to develop the tissue from which they originate. One type of adult stem cell is the epithelial stem cell, which gives rise to the keratinocytes in the multiple layers of epithelial cells in the epidermis of the skin. Adult bone marrow has three distinct types of stem cells:...
4.5K