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High-frequency Ultrasound Imaging of Mouse Cervical Lymph Nodes
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Imaging the Lymph Node Stroma

Clément Ghigo1, Rebecca Gentek1, Marc Bajénoff2

  • 1Aix-Marseille University, Centre National de la Recherche Scientifique (CNRS), Institut National de la Santé et de la Recherche Médicale (INSERM), Centre d'Immunologie de Marseille-Luminy (CIML), Marseille, France.

Methods in Molecular Biology (Clifton, N.J.)
|February 25, 2018
PubMed

Insights

Lymph node stromal cells organize the immune system within complex 3D networks. New high-resolution confocal imaging methods allow detailed in situ analysis of these crucial cells and their functions.

Area of Science:

  • Immunology
  • Cell Biology
  • Microscopy

Background:

  • Lymph node (LN) stromal cells are increasingly recognized for their critical roles in immune system organization.
  • Understanding the in situ functions of LN stromal cells requires studying their complex 3D network architecture.

Purpose of the Study:

  • To describe novel, high-resolution imaging techniques for analyzing lymph node stromal cells.
  • To enable a deeper understanding of the in situ functions of lymph node stromal cells.

Main Methods:

  • Development of two distinct, complementary procedures for sample preparation and high-resolution imaging.
  • Utilizing confocal microscopy to visualize the intricate 3D networks of LN stromal cells.

Main Results:

  • Detailed visualization of lymph node stromal cell architecture at high resolution.
  • Establishment of reliable protocols for in situ analysis of these cells.

Conclusions:

  • The described methods provide powerful tools for studying lymph node stromal cell biology.
  • High-resolution in situ imaging is essential for fully elucidating the functions of LN stromal cells in immunity.

Keywords:
3D reconstructionConfocal imagingCryostat sectioningLymph nodeStromaVibratome sectioning

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