Related Experiment Video

Updated: Feb 12, 2026

Assay of Adhesion Under Shear Stress for the Study of T Lymphocyte-Adhesion Molecule Interactions
07:40

Assay of Adhesion Under Shear Stress for the Study of T Lymphocyte-Adhesion Molecule Interactions

Published on: June 29, 2016

9.1K

Switching between individual and collective motility in B lymphocytes is controlled by cell-matrix adhesion and

Javier Rey-Barroso1,2,3, Daniel S Calovi4, Maud Combe4

  • 1INSERM, UMR1043, Centre de Physiopathologie de Toulouse Purpan, Toulouse, France.

Scientific Reports
|April 13, 2018
PubMed

Insights

B lymphocytes exhibit adaptable movement and clustering behaviors influenced by their environment. Cell motility and group dynamics are regulated by cell-matrix and cell-cell interactions.

Area of Science:

  • Immunology
  • Cell Biology
  • Biophysics

Background:

  • Lymphocytes exhibit dynamic migration and clustering behaviors crucial for immune responses.
  • The factors governing lymphocyte motility and collective behaviors are not fully understood.

Purpose of the Study:

  • To investigate the factors influencing B lymphocyte migration and clustering.
  • To characterize the adaptive motility of B lymphocytes in response to extracellular matrix composition and cell density.

Main Methods:

  • Utilized a model B cell line and primary human B lymphocytes.
  • Collected extensive data on cell displacements, cell-matrix, and cell-cell interactions.
  • Analyzed motility patterns under varying extracellular matrix conditions (collagen IV, fibronectin) and cell densities.

Main Results:

  • Individual B lymphocytes show heterogeneous motility, adapting speed and diffusion based on the extracellular matrix.
  • Collagen IV promotes B lymphocyte clustering and coordinated collective motion at high densities.
  • Fibronectin enhances individual B lymphocyte motility regardless of density.
  • Acto-myosin and Arp2/3 actin dynamics control this behavioral plasticity.

Conclusions:

  • B lymphocyte motility and group dynamics are adaptive, plastic behaviors.
  • These behaviors are intricately regulated by the interplay of cell-matrix interactions and cell-cell interactions.
  • Understanding these dynamics is key to comprehending immune cell function and tissue infiltration.

More Related Videos

Using Cell-substrate Impedance and Live Cell Imaging to Measure Real-time Changes in Cellular Adhesion and De-adhesion Induced by Matrix Modification09:11

Using Cell-substrate Impedance and Live Cell Imaging to Measure Real-time Changes in Cellular Adhesion and De-adhesion Induced by Matrix Modification

Published on: February 19, 2015

11.4K
Static Adhesion Assay for the Study of Integrin Activation in T Lymphocytes09:14

Static Adhesion Assay for the Study of Integrin Activation in T Lymphocytes

Published on: June 13, 2014

16.8K

Related Concept Videos

Overview of Cell-Matrix Interactions01:24

Overview of Cell-Matrix Interactions

The extracellular matrix or ECM holds cells together to form a tissue and allows the cells within the tissue to communicate. ECM comprises proteins such as fibronectin, collagen, laminin, etc. The most abundant protein in this space is collagen. Collagen fibers are interwoven with carbohydrate-containing protein molecules called proteoglycans. ECM allows cell migration and provides a structural scaffold at cell adhesion that anchors the cell when the extracellular matrix proteins interact with...
9.1K
The Extracellular Matrix01:42

The Extracellular Matrix

Overview
89.4K
Adhesion01:14

Adhesion

Adhesion occurs when one type of molecule is attracted to a different molecule. Water exhibits adhesive properties in the presence of polar surfaces, such as glass or cellulose in plants. For instance, when water is poured into a glass, the positively charged hydrogen molecules of water are more attracted to the negatively charged oxygen molecules in the silica than to the oxygen in neighboring water molecules.
Capillary action is a result of water’s adhesive tendencies. When a narrow...
44.6K
Switching of BJT01:22

Switching of BJT

Switching behavior in Bipolar Junction Transistors (BJTs) is a fundamental aspect utilized in various electronic circuits, particularly for digital logic applications like switches and amplifiers. In a typical switching circuit, a BJT alternates between cut-off and saturation modes, corresponding to the "off" and "on" states, respectively, thus behaving like an ideal switch.
Cut-off Mode ("Off" State): In this state, both the emitter-base and collector-base junctions are...
876
Cell Adhesion in Plants01:14

Cell Adhesion in Plants

Plants have rigid cell walls that are made up of cell wall polysaccharides that mediate cell-cell adhesion. The primary cell walls of plants consist of two independent and interacting polysaccharide networks: a pectin matrix that embeds the second network comprising cellulose and hemicelluloses.
Pectins are complex heteropolymers mainly composed of negatively-charged α-D-glucopyranosyl uronic acid and some neutral glycosyl residues such as α-L-rhamnopyranose, α-L-arabinofuranose,...
3.4K
Cell Motility through Blebbing01:16

Cell Motility through Blebbing

Blebs are a type of membrane protrusion formed by the internal hydrostatic pressure of the cytoplasm. Blebs are observed in several cell types, including fibroblasts, immune cells, and single-celled organisms like the amoeba. The primary function of blebs is cell locomotion and apoptosis, but they are also found during necrosis and cell division. The life cycle of a bleb comprises an initiation phase followed by the expansion and retraction phases.
Blebbing Through the Matrix
In multicellular...
2.6K