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Bead Based Multiplex Assay for Analysis of Tear Cytokine Profiles
09:22

Bead Based Multiplex Assay for Analysis of Tear Cytokine Profiles

Published on: October 13, 2017

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Survey of cytokines in normal canine tears by multiplex analysis: A pilot study

Paoul S Martinez1, Eric S Storey1, Cherie M Pucheu-Haston1

  • 1Department of Veterinary Clinical Sciences, School of Veterinary Medicine Louisiana State University, Skip Bertman Drive, Baton Rouge, LA, 70803, United States.

Veterinary Immunology and Immunopathology
|June 20, 2018
PubMed

Insights

This pilot study measured cytokines in canine tears using a multiplex assay. Interleukin-8 (IL-8) was consistently detected, suggesting undiluted samples are best for canine tear cytokine analysis.

Area of Science:

  • Veterinary Ophthalmology
  • Immunology
  • Biochemistry

Background:

  • Tear cytokines play crucial roles in ocular surface health and disease.
  • Limited research exists on canine tear cytokine profiles, hindering diagnostic and therapeutic advancements.

Purpose of the Study:

  • To quantify cytokine levels in normal stimulated canine tears using a multiplexed assay.
  • To establish baseline cytokine concentrations for future comparative studies in canine ocular conditions.

Main Methods:

  • Stimulated tears were collected from 25 healthy dogs and stored at -80°C.
  • A Luminex multiplex assay analyzed tears for IL-2, IL-6, IL-7, IL-8, IL-10, TNF-α, and IFN-γ.
  • Samples were initially tested at a 1:10 dilution, with eight re-analyzed undiluted.

Main Results:

  • A 1:10 dilution rendered most analytes undetectable, except for IL-8.
  • Undiluted samples revealed consistent detection of IL-8 (16/16 eyes) and IFN-γ (15/16 eyes).
  • IL-8 was significantly higher in undiluted samples compared to IFN-γ.

Conclusions:

  • This is the first study to report on cytokine analysis in canine tears.
  • Interleukin-8 (IL-8) is reliably detected in canine tears, regardless of dilution.
  • Undiluted canine tear samples are recommended for accurate cytokine analysis, particularly for IFN-γ.
Abstract

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