Related Experiment Video

Updated: Feb 8, 2026

Stimulation of Notch Signaling in Mouse Osteoclast Precursors
08:01

Stimulation of Notch Signaling in Mouse Osteoclast Precursors

Published on: February 28, 2017

8.4K

Notch Signaling Facilitates In Vitro Generation of Cross-Presenting Classical Dendritic Cells

Margaret E Kirkling1, Urszula Cytlak2, Colleen M Lau3

  • 1Department of Pathology, New York University School of Medicine, New York, NY 10016, USA; Graduate Program in Genetics and Development, Columbia University Medical Center, New York, NY 10032, USA.

Cell Reports
|June 21, 2018
PubMed

Insights

Notch signaling via OP9-DL1 co-culture significantly enhances the generation of dendritic cell 1 (cDC1s) from hematopoietic progenitors. This method yields functional cDC1s crucial for robust T cell responses against tumors and pathogens.

Area of Science:

  • Immunology
  • Cell Biology
  • Developmental Biology

Background:

  • Classical dendritic cells (cDC1s) are vital for initiating cytotoxic T cell responses against pathogens and tumors.
  • Current methods using FLT3 ligand (FLT3L) produce limited numbers of cDC1s or immature cells.

Purpose of the Study:

  • To optimize the in vitro generation of functional cDC1s.
  • To improve the yield of cDC1s for research and potential therapeutic applications.

Main Methods:

  • Co-culture of murine immortalized progenitors and primary bone marrow cells with OP9 stromal cells expressing Delta-like 1 (OP9-DL1) and FLT3L.
  • Phenotypic and expression profiling of generated cDC1s.
  • Assessment of in vitro migration and in vivo T cell cross-priming and antitumor efficacy.

Main Results:

  • OP9-DL1 co-culture significantly increased the generation of IRF8-dependent cDC1s with a phenotype and expression profile similar to primary splenic cDC1s.
  • OP9-DL1-induced cDC1s exhibited enhanced migration towards CCR7 ligands in vitro.
  • These cDC1s demonstrated superior T cell cross-priming and antitumor vaccination effects in vivo.
  • The method also substantially improved the yield of human CD141+ cDC1s.

Conclusions:

  • Notch signaling, through OP9-DL1, is a key factor in optimizing dendritic cell generation in vitro.
  • This approach provides a reliable method for producing authentic cDC1s for functional studies and translational applications.

More Related Videos

Novel Protocol for Generating Physiologic Immunogenic Dendritic Cells12:08

Novel Protocol for Generating Physiologic Immunogenic Dendritic Cells

Published on: May 17, 2019

9.7K
Real-time Bioluminescence Imaging of Notch Signaling Dynamics during Murine Neurogenesis10:25

Real-time Bioluminescence Imaging of Notch Signaling Dynamics during Murine Neurogenesis

Published on: December 12, 2019

8.2K

Related Concept Videos

Notch Signaling Pathway03:14

Notch Signaling Pathway

The Notch signaling pathway is a major intracellular signaling pathway that is highly conserved over a broad spectrum of metazoan species. It stands unique from other intracellular signaling mechanisms in animals because notch protein itself acts as the receptor as well as the primary signaling molecule.
The Notch gene came into the limelight in 1914 after the discovery that its mutation in Drosophila melanogaster leads to a serrated (or "notched") wing margin phenotype. It was not...
6.6K
Role Of Notch Signalling In Intestinal Stem Cell Renewal01:12

Role Of Notch Signalling In Intestinal Stem Cell Renewal

Notch signaling was first discovered in Drosophila melanogaster, where it is involved in cell lineage differentiation. Notch signaling regulates the maintenance and differentiation of intestinal stem cells or ISCs by controlling the expression of atonal homolog 1 or Atoh1. Atoh1 directs cells to differentiate into secretory cells.
Direct cell-to-cell contact is needed for the activation of Notch signaling. The signal is initiated when a notch ligand binds to a receptor on an adjacent cell, also...
2.5K
Facilitated Transport01:19

Facilitated Transport

The chemical and physical properties of plasma membranes cause them to be selectively permeable. Since plasma membranes have both hydrophobic and hydrophilic regions, substances need to be able to transverse both regions. The hydrophobic area of membranes repels substances such as charged ions. Therefore, such substances need special membrane proteins to cross a membrane successfully. In  facilitated transport, also known as facilitated diffusion, molecules and ions travel across a...
149.7K
What is Cell Signaling?02:03

What is Cell Signaling?

Despite the protective membrane that separates a cell from the environment, cells need the ability to detect and respond to environmental changes. Additionally, cells often need to communicate with one another. Unicellular and multicellular organisms use a variety of cell signaling mechanisms to communicate to respond to the environment.
131.0K
Crossing Over01:34

Crossing Over

Unlike mitosis, meiosis aims for genetic diversity in its creation of haploid gametes. Dividing germ cells first begin this process in prophase I, where each chromosome—replicated in S phase—is now composed of two sister chromatids (identical copies) joined centrally.
The homologous pairs of sister chromosomes—one from the maternal and one from the paternal genome—then begin to align alongside each other lengthwise, matching corresponding DNA positions in a process...
172.2K
Classical Conditioning01:18

Classical Conditioning

Associative learning, a core principle in behavioral psychology, involves forming connections between events and facilitating learned responses. This concept is vividly illustrated by classical conditioning, a process extensively studied by the Russian physiologist Ivan Pavlov. Pavlov's pioneering research on dogs' digestive systems led to the discovery that behaviors can be learned through association, laying the groundwork for classical conditioning.
Ivan Pavlov observed that dogs...
2.3K