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Transcription factor c-Maf is essential for IL-10 gene expression in B cells

Min Liu1, Xiaoqi Zhao2, Yunfeng Ma3

  • 1Department of Immunology, School of Medicine, Wuhan University, Wuhan, China.

Scandinavian Journal of Immunology
|July 6, 2018
PubMed

Insights

The transcription factor c-Maf is crucial for producing interleukin-10 (IL-10) in B cells. Its reduced expression in collagen-induced arthritis models suggests a role in disease pathology.

Area of Science:

  • Immunology
  • Molecular Biology
  • Cell Biology

Background:

  • Interleukin-10 (IL-10) is a key anti-inflammatory cytokine regulating immune responses.
  • c-Maf is a known transcriptional factor for IL-10 in T cells and macrophages.
  • The role of c-Maf in IL-10 production within B cells is not well understood.

Purpose of the Study:

  • To investigate the function of c-Maf in the transcriptional regulation of IL-10 in regulatory B cells.
  • To elucidate the molecular mechanisms underlying c-Maf's role in IL-10 expression in B cells.

Main Methods:

  • Analysis of c-Maf expression in resting and lipopolysaccharide (LPS)-activated B cells.
  • Investigation of c-Maf binding to the IL-10 promoter.
  • Assessment of c-Maf and regulatory B cell levels in a collagen-induced arthritis (CIA) mouse model.

Main Results:

  • c-Maf is constitutively expressed in resting B cells.
  • LPS stimulation upregulates c-Maf expression and enhances IL-10 production via promoter binding.
  • Reduced c-Maf and regulatory B cell populations were observed in CIA mice.

Conclusions:

  • c-Maf is an essential transcription factor for IL-10 gene expression in LPS-activated B cells.
  • The findings suggest c-Maf's involvement in the pathogenesis of conditions like CIA.

Keywords:
BregIL-10c-Mafcollagen-induced arthritis

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