Related Experiment Video

Updated: Feb 8, 2026

Isolation of Myeloid Dendritic Cells and Epithelial Cells from Human Thymus
09:07

Isolation of Myeloid Dendritic Cells and Epithelial Cells from Human Thymus

Published on: September 19, 2013

19.9K

Isolation and Characterization of Human Lung Myeloid Cells

Yen-Rei A Yu1, Robert M Tighe2

  • 1Department of Medicine, Division of Pulmonary and Critical Care Medicine, Duke University School of Medicine, Durham, NC, USA. yenrei.yu@duke.edu.

Methods in Molecular Biology (Clifton, N.J.)
|July 11, 2018
PubMed

Insights

This study presents a novel multiparameter flow cytometry method for analyzing human lung immune cells. The approach uses a base antibody panel to identify major immune cell types in lung tissue, blood, and bronchoalveolar lavage fluid.

Area of Science:

  • Immunology
  • Pulmonology
  • Cell Biology

Background:

  • Characterizing pulmonary immune cells is crucial for understanding lung health and disease.
  • Existing methods for immune cell analysis can be complex and time-consuming.

Purpose of the Study:

  • To develop a standardized approach for multiparameter flow cytometry of human lung immune cells.
  • To establish a base antibody panel for comprehensive immune cell identification.

Main Methods:

  • Processing of human lung tissues for flow cytometry.
  • Utilization of a defined base antibody panel for single-staining conditions.
  • Application of the panel to human blood and bronchoalveolar lavage (BAL) fluid.

Main Results:

  • Successful characterization of immune cell composition, phenotype, and function in human lungs.
  • Identification of all major immune cell types within a single staining protocol.
  • Validation of the panel's utility in blood and BAL fluid samples.

Conclusions:

  • The described flow cytometry approach enables efficient and comprehensive analysis of pulmonary immune cells.
  • The base antibody panel provides a versatile tool for immune cell identification across different human sample types.
  • This method facilitates further research into lung immunity and related pathologies.

Keywords:
Bronchoalveolar lavageDendritic cellsFlow cytometryGranulocytesImmune cellsImmunophenotypingLymphocytesMacrophagesMonocytesMononuclear phagocytes

More Related Videos

Isolation of Live Myeloid and Epithelial Cell Populations from the Mouse Lung06:02

Isolation of Live Myeloid and Epithelial Cell Populations from the Mouse Lung

Published on: January 31, 2025

1.8K
Isolation and Enrichment of Human Lung Epithelial Progenitor Cells for Organoid Culture11:49

Isolation and Enrichment of Human Lung Epithelial Progenitor Cells for Organoid Culture

Published on: July 21, 2020

9.1K

Related Concept Videos

Differentiation of Common Myeloid Progenitor Cells01:15

Differentiation of Common Myeloid Progenitor Cells

Common myeloid progenitors (CMPs) are oligopotent cells that can differentiate into granulocytes and macrophages. Granulocytes and macrophages are essential for protecting the body against bacterial, viral, or fungal infections. They migrate from the bone marrow into the circulating blood to reach specific tissue sites where they differentiate and help in immune surveillance. However, they survive only for a few days and must be continuously made available to the organism to maintain a robust...
4.0K
Lung Capacity01:47

Lung Capacity

The air in the lungs is measured in volumes and capacities. Lung volume measures reflect the amount of air taken in, released, or left over after a lung function, like a single inhalation. Lung capacity measures are sums of two or more lung volume measures.
56.4K
Pleura of the Lungs01:13

Pleura of the Lungs

The lungs are nestled in a cavity, shielded by the pleura. The pleura, a form of serous membrane, wraps around each lung. This membrane arrangement consists of two layers: the visceral and parietal pleurae. The visceral pleura lines the surface of the lungIn contrast, the parietal pleura is the outer layer and contacts to the thoracic wall, the mediastinum, and the diaphragm. The hilum is the point of connection between the visceral and parietal layers. The space between the parietal and...
7.4K
Overview Of Cell Separation And Isolation01:20

Overview Of Cell Separation And Isolation

Cell separation was first achieved in 1964 by S. H. Seal, who separated large tumor cells from the smaller blood cells using filtration. Two years later, Pohl and Hawk performed experiments on how cells respond differently to a nonuniform electric field based on the cell type. Such observations were the inception of cell separation methods, which allow isolating a single cell type from a heterogeneous sample.
7.6K
Gross Anatomy of the Lungs01:17

Gross Anatomy of the Lungs

The lungs are a pair of vital organs connected to the trachea via the left and right bronchi. The base of these organs meets the dome-shaped muscle known as the diaphragm. Encased by the pleurae, the lungs contact the mediastinum. The right lung is shorter yet wider, and has a larger volume than the left lung. The left lung has an indentation known as the cardiac notch. The superior region of the lungs is referred to as the apex, whereas the base is the lower region near the diaphragm. The...
4.8K
Biodiversity and Human Values01:24

Biodiversity and Human Values

Human civilization relies on biodiversity in many ways. Sudden changes in species biodiversity result in environmental changes that can modify weather patterns and therefore human civilizations.
17.1K