Related Experiment Video

Updated: Feb 7, 2026

Detection of Extravascular Trypanosoma Parasites by Fine Needle Aspiration
08:33

Detection of Extravascular Trypanosoma Parasites by Fine Needle Aspiration

Published on: August 7, 2019

9.6K

Expression of p63 immunostaining in liquid-based cytology (BD SurePath) of breast fine-needle aspiration

Masumi Osugi1, Kosei Kinoshita1, Atsuro Sugita1

  • 1Department of Pathology, Ehime Prefectural Central Hospital, Matsuyama, Ehime, Japan.

Diagnostic Cytopathology
|July 26, 2018
PubMed

Insights

Adding p63 immunostaining to liquid-based cytology (LBC) significantly improves breast fine-needle aspiration cytology (FNAC) diagnostic accuracy. This method enhances the detection of myoepithelial cells, leading to more reliable results in breast lesion diagnosis.

Area of Science:

  • Pathology
  • Cytopathology
  • Oncology

Background:

  • Myoepithelial cells play a crucial role in breast lesion diagnosis.
  • Traditional methods for detecting myoepithelial cells in breast fine-needle aspiration cytology (FNAC) can be limited.
  • Liquid-based cytology (LBC) offers a potential platform for improved cellular preservation and analysis.

Purpose of the Study:

  • To evaluate the impact of p63 immunostaining on the diagnostic accuracy of breast FNAC using BD SurePath LBC slides.
  • To determine if p63 immunostaining enhances the detection of myoepithelial cells for improved breast lesion classification.

Main Methods:

  • p63 immunostaining was performed on BD SurePath liquid-based cytology (LBC) slides.
  • The study analyzed 298 breast lesions diagnosed via FNAC between July 2010 and August 2016.
  • Diagnostic accuracy metrics were compared between conventional Papanicolaou staining and LBC with p63 immunostaining.

Main Results:

  • Specific cutoff values for malignancy were established based on p63+ cell percentages and counts.
  • Malignant lesions demonstrated significantly lower percentages of p63+ clusters and single cells compared to benign lesions (P < .001).
  • LBC-p63 immunostaining significantly improved sensitivity (88.3% to 99.0%), specificity (89.1% to 99.0%), and overall diagnostic accuracy (88.6% to 99.0%) compared to Papanicolaou staining alone.

Conclusions:

  • The addition of LBC-p63 immunostaining significantly enhances the diagnostic accuracy of breast FNAC.
  • This technique provides a more reliable method for identifying myoepithelial cells, aiding in the differentiation of benign and malignant breast lesions.
Abstract

Keywords:
breast pitfallfine-needle aspiration cytologyliquid-based cytologymyoepithelial cellp63 immunocytochemistry

More Related Videos

Preparation and Using Phantom Lesions to Practice Fine Needle Aspiration Biopsies09:26

Preparation and Using Phantom Lesions to Practice Fine Needle Aspiration Biopsies

Published on: September 29, 2009

18.6K
Use of Electromagnetic Navigational Transthoracic Needle Aspiration E-TTNA for Sampling of Lung Nodules06:03

Use of Electromagnetic Navigational Transthoracic Needle Aspiration E-TTNA for Sampling of Lung Nodules

Published on: May 23, 2015

22.9K

Related Concept Videos

Fineness of Cement01:15

Fineness of Cement

The fineness of cement directly influences the rate of hydration, as the hydration begins at the surface of the cement particles. In addition to hydration, the fineness of cement is vital for various properties of concrete including workability, gypsum requirement, and long-term behavior. The fineness of cement is represented in terms of the specific surface of cement which is typically measured in square meters per kilogram, with several methods available for this determination.
Direct...
522
Fineness Modulus01:19

Fineness Modulus

The fineness modulus (FM) of aggregate is a numerical index that measures the coarseness or fineness of the particles. It is calculated by adding the cumulative percentages of aggregate retained on each of a specified series of sieves and dividing the sum by 100.
Consider performing sieve analysis on sand through a set of ASTM sieves. The weight of aggregate retained in each sieve and pan placed at the bottom is recorded, as given in Column B of Table 1.
To determine the fineness modulus of...
1.5K
What is Gene Expression?01:42

What is Gene Expression?

Overview
Gene expression is the process in which DNA directs the synthesis of functional products, that is, proteins. Cells can regulate gene expression at various stages. It allows organisms to generate different cell types and enables cells to adapt to internal and external factors.
Genetic Information Flows from DNA to RNA to Protein
A gene is a stretch of DNA that serves as the blueprint for functional RNAs and proteins. Since DNA is made up of nucleotides and proteins consist of amino...
196.9K
Molecular Comparison of Gases, Liquids, and Solids02:26

Molecular Comparison of Gases, Liquids, and Solids

Particles in a solid are tightly packed together (fixed shape) and often arranged in a regular pattern; in a liquid, they are close together with no regular arrangement (no fixed shape); in a gas, they are far apart with no regular arrangement (no fixed shape). Particles in a solid vibrate about fixed positions (cannot flow) and do not generally move in relation to one another; in a liquid, they move past each other (can flow) but remain in essentially constant contact; in a gas, they move...
55.3K
Sputum Studies I: Gram Stain, cytology, and Acid-fast smear and culture01:26

Sputum Studies I: Gram Stain, cytology, and Acid-fast smear and culture

Sputum studies are a critical part of diagnosing and treating numerous respiratory conditions. These studies involve obtaining sputum samples for analysis to identify pathogenic organisms and assess the presence of abnormal cells indicative of malignant conditions. This lesson will delve into three fundamental sputum studies: Gram Stain, Cytology, and Acid-fast Smear and Culture.
Gram Stain
The Gram Stain is an integral part of sputum studies. It involves the staining of sputum, which permits...
684
Rise of Liquid in a Capillary Tube01:18

Rise of Liquid in a Capillary Tube

When very thin cylindrical tubes, called capillaries, are dipped in a liquid, the liquid rises or falls in the tube compared to the surrounding liquid. This phenomenon is called capillary action. Capillary action occurs due to the combination of two opposing forces: the cohesive forces of the liquid, which cause it to stick to itself and form a rounded shape, and the adhesive forces between the liquid and the walls of the container, which cause the liquid to be attracted to the container walls.
3.2K