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PGK1 as an immune target in Kawasaki disease
1112 Laboratory, School of Chemistry and Biological Engineering, University of Science and Technology Beijing, Beijing, China.
Insights
Researchers identified phosphoglycerate kinase 1 (PGK1) as a potential immune target in Kawasaki disease (KD). This discovery may aid in understanding KD pathogenesis and improve laboratory diagnosis for this serious childhood illness.
Area of Science:
- Immunology
- Molecular Biology
- Pediatric Rheumatology
Background:
- Kawasaki disease (KD) is an immune-mediated vasculitis presenting with prolonged fever.
- The precise immune origins and diagnostic markers for KD remain incompletely understood.
Purpose of the Study:
- To identify specific immune targets involved in Kawasaki disease pathogenesis.
- To evaluate the diagnostic potential of identified targets for KD.
Main Methods:
- Utilized cell chip technology to screen for immune targets in KD patient sera.
- Identified phosphoglycerate kinase 1 (PGK1) as a target antigen using immunofluorescence and proteomic analysis.
- Validated PGK1 as an antigen using ELISA, Western blotting, and immunoprecipitation.
Main Results:
- Immunoglobulin (Ig)G antibodies against PGK1 were detected in 46% of KD patients.
- Lower prevalence of anti-PGK1 antibodies was observed in febrile non-KD patients (13%) and healthy donors (2.6%).
- PGK1 demonstrated significant antigen recognition by KD patient sera.
Conclusions:
- Phosphoglycerate kinase 1 (PGK1) is identified as a potential immune target in Kawasaki disease.
- PGK1 holds promise as a biomarker for improving the laboratory diagnosis of KD.
- Further research into PGK1's role can advance understanding of KD pathogenesis.
Abstract:
Kawasaki disease (KD) is an immune-mediated vasculitis with symptoms that mimic febrile illness; the immune origin has been suggested but never proved. First, cell chip technology was used to screen immune targets cells. With the indirect immunofluorescence assay we found that the HeLa cell chip could be recognized by KD patient serum samples. The target cell proteome was extracted as antigens, and antigen recognition reaction was performed using the patients' serum as antibodies and the detected target protein was detected and identified as phosphoglycerate kinase 1 (PGK1). Then PGK1 was produced and tested with enzyme-linked immunosorbent assay (ELISA), Western blotting, immunoprecipitation and competitive inhibition immunofluorescence assay. Immunoglobulin (Ig)G against PGK1 was detected in 46% (23 of 50) sera of KD patients, 13% (five of 38) sera in febrile non-KD patients (FC) and 2·6% (one of 38) sera in healthy donors. As an immune target, PGK1 not only helps understanding of the pathogenesis, it also has potential value in facilitating the laboratory diagnosis of KD.
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