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[Sorting and identification of human immature Langerhans cells]

Jianbin Wang1, Lichun Wang1, Lei Liu1

  • 1Yunnan Key Laboratory of Vaccine Research and Development on Severe Infectious Disease; Institute of Medical Biology, Chinese Academy of Medical Sciences and Peking Union Medical College, Kunming 650118, China.

Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi = Chinese Journal of Cellular and Molecular Immunology
|November 2, 2018
PubMed

Insights

Researchers developed an effective method to isolate immature Langerhans cells (LCs) from human skin. This technique successfully identified and harvested these crucial immune cells for further study.

Area of Science:

  • Dermatology
  • Immunology
  • Cell Biology

Background:

  • Langerhans cells (LCs) are critical immune cells residing in the human epidermis.
  • Understanding LCs requires methods for their isolation and phenotypic identification.
  • Immature LCs play a vital role in immune surveillance and response.

Purpose of the Study:

  • To establish a reliable protocol for isolating immature Langerhans cells (LCs) from human foreskin epidermis.
  • To characterize the phenotype of isolated LCs using surface markers.
  • To assess the viability of the sorted immature LCs.

Main Methods:

  • Human foreskin tissue was treated with dispase I and collagenase I to obtain epidermal cell suspension.
  • Immature LCs were isolated using flow cytometry, targeting CD3-, CD14-, CD1a+, and CD45+ markers.
  • Phenotypic identification was confirmed via immunofluorescence assay for CD207, and cell viability was assessed using trypan blue staining.

Main Results:

  • A population of immature Langerhans cells (LCs) was successfully sorted, achieving 91.31% purity.
  • The isolated LCs exhibited a high survival rate of 93.16% post-sorting.
  • The study confirmed the effectiveness of the isolation and identification procedure.

Conclusions:

  • A robust and effective technical procedure for sorting immature Langerhans cells (LCs) from human foreskin has been established.
  • This method allows for the isolation of a highly pure population of viable immature LCs.
  • The established protocol facilitates further research into the function and role of immature LCs in skin immunity.

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