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Visualization, Quantification, and Mapping of Immune Cell Populations in the Tumor Microenvironment
11:00

Visualization, Quantification, and Mapping of Immune Cell Populations in the Tumor Microenvironment

Published on: March 25, 2020

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Determination and Isolation of Immune Populations from Brain Tumor Microenvironments

Javier Godino1, Alberto J Schuhmacher2

  • 1Cell Separation and Flow Cytometry Core Facility, Aragon Institute of Health Sciences (IACS), Zaragoza, Spain.

Methods in Molecular Biology (Clifton, N.J.)
|November 23, 2018
PubMed

Insights

This study details a method for analyzing mouse brain tumors using flow cytometry. This technique isolates and characterizes various immune cells within the tumor microenvironment.

Area of Science:

  • Immunology
  • Oncology
  • Biotechnology

Background:

  • Flow cytometry and fluorescence-activated cell sorting (FACS) are crucial for cell analysis.
  • Understanding the immune cell composition of tumors is vital for cancer research.

Purpose of the Study:

  • To describe a method for preparing and analyzing single-cell suspensions from mouse brain tumors.
  • To characterize and isolate distinct immune cell populations within the tumor microenvironment.

Main Methods:

  • Single cell suspension preparation from freshly dissected mouse brain tumors.
  • Staining with fluorescently labeled antibodies.
  • Flow cytometry analysis for cell determination, characterization, and isolation.

Main Results:

  • Successful preparation of single-cell suspensions from mouse brain tumors.
  • Identification and characterization of diverse immune cell populations.
  • Isolation of specific immune cell subsets for further study.

Conclusions:

  • The described method enables detailed analysis of immune infiltrates in brain tumors.
  • This approach facilitates the study of tumor immunology and the development of targeted therapies.

Keywords:
AntibodyFlow cytometryFluorescence-activated cell sorting (FACS)FluorophoreImmune cell infiltrateTumor microenvironment

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