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Recruitment and Collection of Dermal Interstitial Fluid Using a Microneedle Patch

Chandana Kolluru1, Mikayla Williams1, Jeremy Chae1

  • 1School of Chemical and Biomolecular Engineering, Georgia Institute of Technology, 311 Ferst Drive, Atlanta, GA, 30332, USA.

Advanced Healthcare Materials
|January 5, 2019
PubMed

Insights

A novel microneedle (MN) patch offers a simple, rapid method for collecting interstitial fluid (ISF) from the skin. This minimally invasive technique yields blood-free ISF for biomarker analysis, aiding future diagnostics.

Area of Science:

  • Biomarker discovery
  • Microneedle technology
  • Fluid dynamics

Background:

  • Interstitial fluid (ISF) is a valuable source of biomarkers.
  • Conventional methods for ISF collection are often invasive or complex.
  • There is a need for simple, rapid, and cost-effective ISF harvesting techniques.

Purpose of the Study:

  • To develop and optimize a minimally invasive microneedle (MN) patch for interstitial fluid (ISF) collection.
  • To evaluate the efficiency and feasibility of the MN patch in vivo.
  • To explore potential applications of MN-based ISF collection in diagnostics and research.

Main Methods:

  • Development of a microneedle (MN) patch with 650 µm long needles.
  • Application of the MN patch to rat skin in vivo at a specific angle.
  • Optimization of ISF collection by varying skin pretreatment and delay times.
  • Quantification of collected ISF volume and assessment of blood-free properties.

Main Results:

  • The MN patch successfully collected >2 µL of blood-free ISF within 1 minute in rat skin.
  • A brief skin pretreatment with MNs followed by a 5-minute delay significantly enhanced ISF collection.
  • The optimized method was well-tolerated and demonstrated high efficiency.
  • The mechanism for enhanced collection is hypothesized to involve increased skin hydration.

Conclusions:

  • A minimally invasive microneedle patch provides a rapid, simple, and cost-effective method for collecting interstitial fluid (ISF).
  • This technique offers a promising alternative to conventional methods for accessing ISF biomarkers.
  • MN-based ISF collection has significant potential for advancing medical diagnostics and monitoring.

Keywords:
biomarkersdermal interstitial fluidmicroneedle patchesskin hydrationtransdermal transport

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