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Updated: Jan 31, 2026

Flow Cytometry to Estimate Leukemia Stem Cells in Primary Acute Myeloid Leukemia and in Patient-derived-xenografts, at Diagnosis and Follow Up
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Flow Cytometry to Estimate Leukemia Stem Cells in Primary Acute Myeloid Leukemia and in Patient-derived-xenografts, at Diagnosis and Follow Up

Published on: March 26, 2018

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Clinically useful flow cytometry approach to identify immunophenotype in acute leukemia

Guifang Ouyang1,2, Zhijuan Xu1,2, Danjie Jiang3,2

  • 11 Department of Hematology, Ningbo First Hospital, Ningbo, Zhejiang, China.

The Journal of International Medical Research
|January 8, 2019
PubMed

Insights

Flow cytometry rapidly identifies immunophenotypes in acute leukemia (AL). The study suggests cluster of differentiation 2 (CD2) may predict prognosis in acute myeloid leukemia (AML) patients.

Area of Science:

  • Hematology
  • Immunology
  • Oncology

Background:

  • Acute leukemia (AL) is a complex malignant disease originating from hematopoietic cell abnormalities.
  • Immunophenotyping is crucial for AL diagnosis and classification.
  • Flow cytometry offers a potential method for rapid immunophenotyping.

Purpose of the Study:

  • To investigate the utility of flow cytometry for immunophenotyping leukemic cells.
  • To assess the clinical usefulness of immunophenotyping in AL treatment.
  • To explore the prognostic value of specific immunophenotypic markers.

Main Methods:

  • Bone marrow specimens from AL patients were analyzed for antigen expression using flow cytometry.
  • Kaplan-Meier survival analysis was employed to evaluate marker impact on prognosis.
  • Genetic testing was performed to detect the BCR-ABL fusion gene.

Main Results:

  • Distinct immunophenotypic marker expression profiles were observed between acute lymphoblastic leukemia (ALL) and acute myeloid leukemia (AML).
  • A significant association between the CD2 surface marker and survival was found in AML, but not in ALL.
  • Increased prevalence of the BCR-ABL fusion gene was noted in ALL compared to AML samples.

Conclusions:

  • Flow cytometry provides a rapid and effective method for AL immunophenotype identification.
  • The CD2 marker shows potential as a predictive prognostic indicator for AML patients.
Abstract

Keywords:
Acute leukemiaCD2acute lymphoblastic leukemiabone marrowbreak point cluster-Abelson tyrosine kinase fusion geneflow cytometryimmunophenotypemyeloid leukemia

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