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Analysis of Tissue-Resident Immune Cells from Mouse Skin and Lungs by Flow Cytometry

Felipe Galvez-Cancino1, Ernesto Lopez1, Alvaro Lladser2

  • 1Laboratory of Gene Immunotherapy, Fundación Ciencia & Vida, Santiago, Chile.

Methods in Molecular Biology (Clifton, N.J.)
|January 23, 2019
PubMed

Insights

Studying immune cells in complex tissues like skin and lungs is challenging. This optimized method enables precise analysis of CD8+ T cells in these nonlymphoid tissues.

Area of Science:

  • Immunology
  • Cell Biology
  • Tissue Engineering

Background:

  • Nonlymphoid tissues present significant heterogeneity and complexity, hindering immune cell population studies.
  • Reproducible protocols are essential for advancing clinical and preclinical research involving immune cells in tissues.

Purpose of the Study:

  • To develop and optimize a method for obtaining single-cell suspensions from skin and lung tissues.
  • To enable accurate analysis and quantification of tissue-resident memory CD8+ T cells.

Main Methods:

  • Development of an optimized protocol for single-cell suspension preparation from skin and lung tissues.
  • Utilizing multi-parametric flow cytometry for immune cell analysis.

Main Results:

  • Successful generation of single-cell suspensions from skin and lung samples.
  • Quantification of tissue-resident memory CD8+ T cell populations using the developed method.

Conclusions:

  • The optimized protocol facilitates the study of immune cells within complex nonlymphoid tissues.
  • This method improves the analysis of tissue-resident memory CD8+ T cells, crucial for immunological research.

Keywords:
Barrier tissuesFlow cytometryImmunophenotypingMemory CD8+ T cellsSingle-cell suspensions

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