Related Experiment Video

Updated: Jan 29, 2026

Author Spotlight: Exploring the Role of Unfolded Protein Response in HIV-1 Replication and Infectivity
10:12

Author Spotlight: Exploring the Role of Unfolded Protein Response in HIV-1 Replication and Infectivity

Published on: June 14, 2024

2.6K

HIV-1 infection modulates IL-24 expression which contributes to cell apoptosis in vitro

Scheilla Teixeira Strumillo1, Marli Ferreira Curcio1, Fábio Ferreira de Carvalho2

  • 1Disciplina de Infectologia, Laboratório de Retrovirologia, Universidade Federal de São Paulo, São Paulo, Brazil.

Cell Biology International
|February 15, 2019
PubMed

Insights

Interleukin-24 (IL-24) gene and protein expression are altered during early HIV-1 infection. Neutralizing IL-24 reduced apoptosis in infected cells, suggesting a role in viral persistence.

Area of Science:

  • Immunology
  • Virology
  • Molecular Biology

Background:

  • Interleukin-24 (IL-24) is implicated in various diseases but its role in HIV-1 infection remains unclear.
  • Understanding IL-24's function in early HIV-1 infection is crucial for developing new therapeutic strategies.

Purpose of the Study:

  • To investigate the gene and protein expression of IL-24 in peripheral blood mononuclear cells (PBMCs) during the initial stages of HIV-1 infection.
  • To assess the impact of IL-24 on apoptosis-related pathways, including caspase-3 and ERK phosphorylation, in HIV-1 infected cells.

Main Methods:

  • Quantitative analysis of IL-24 gene and protein expression in PBMCs.
  • Flow cytometry using Annexin V/Propidium Iodide staining to evaluate apoptosis.
  • Western blot analysis for caspase-3 and phosphorylated ERK (p-ERK) expression.
  • Incubation with IL-24 neutralizing antibody to assess its effect on apoptosis.

Main Results:

  • HIV-1 infection significantly affected the gene and protein expression levels of IL-24 and ERK.
  • Annexin V/Propidium Iodide assays showed a reduction in apoptotic mechanisms in HIV-1 infected cells following IL-24 neutralization.
  • IL-24 plays a role in modulating apoptosis during early HIV-1 infection.

Conclusions:

  • HIV-1 infection influences IL-24 expression and its associated apoptotic signaling pathways.
  • Targeting IL-24 may offer a novel approach for antiretroviral strategies by modulating viral persistence mechanisms.

Keywords:
HIV-1IL-24 expressionapoptosis

More Related Videos

In vitro Uncoating of HIV-1 Cores10:49

In vitro Uncoating of HIV-1 Cores

Published on: November 8, 2011

16.3K
New Tools to Expand Regulatory T Cells from HIV-1-infected Individuals09:27

New Tools to Expand Regulatory T Cells from HIV-1-infected Individuals

Published on: May 30, 2013

11.6K

Related Concept Videos

Cell Specific Gene Expression01:58

Cell Specific Gene Expression

Multicellular organisms contain a variety of structurally and functionally distinct cell types, but the DNA in all the cells originated from the same parent cells. The differences in the cells can be attributed to the differential gene expression. Liver cells, whose functions include detoxification of blood, production of bile to metabolize fats, and synthesis of proteins essential for metabolism, must express a specific set of genes to perform their functions. Gene expression also varies with...
16.5K
Cell Specific Gene Expression01:58

Cell Specific Gene Expression

5.6K
Apoptosis01:30

Apoptosis

Apoptosis is a combination of two Greek words, 'apo' and 'ptosis,' meaning separation and falling off, respectively. Hippocrates used this word to describe gangrene, which was caused due to bandaging of fractured bones. Apoptosis was distinguished from necrosis in 1970 when John Kerr reported observations of morphological changes occurring during apoptosis. During one experiment, he observed that the disruption of blood supply to the liver tissue resulted in a size...
14.4K
Binet's Contribution to Measures of Intelligence01:23

Binet's Contribution to Measures of Intelligence

Alfred Binet, along with his student Théophile Simon, was tasked by the French Ministry of Education in 1904 to create a method for identifying students who struggled to learn through conventional classroom instruction. This initiative aimed to address overcrowding by placing such students in specialized schools. Binet and Simon developed an intelligence test comprising 30 tasks, ranging from simple commands, like touching one's nose or ear, to more complex tasks, such as drawing...
1.7K
Holter Monitor: 24-Hour Monitoring01:23

Holter Monitor: 24-Hour Monitoring

Holter monitoring is a continuous electrocardiography (ECG) recording that tracks the heart's electrical activity over an extended period, generally 24 to 48 hours. This noninvasive diagnostic tool detects irregular heart rhythms that may not be captured during a standard ECG performed in a clinical setting.DeviceThe Holter monitor is a portable, small device connected to several electrodes on the patient's chest. These electrodes detect the heart's electrical signals and transmit them to the...
2.4K
Wechsler's Contribution to Measures of Intelligence01:23

Wechsler's Contribution to Measures of Intelligence

David Wechsler, a psychologist who worked with World War I veterans, developed a significant IQ test in 1939 called the Wechsler-Bellevue Intelligence Scale. This test was innovative because it combined several subtests that measured both verbal and nonverbal skills, reflecting Wechsler's belief that intelligence is a global capacity involving purposeful action, rational thinking, and effective interaction with the environment. This test later evolved into the Wechsler Adult Intelligence...
2.1K