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Synthetic Antigen Controls for Immunohistochemistry
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Synthetic Antigen Controls for Immunohistochemistry

Published on: August 23, 2021

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Synthetic Antigen Gels as Practical Controls for Standardized and Quantitative Immunohistochemistry

Kathy J Hötzel1, Charles A Havnar1, Hai V Ngu1

  • 1Department of Research Pathology, Genentech, Inc., South San Francisco, California.

The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society
|March 19, 2019
PubMed

Insights

Reproducible immunohistochemistry (IHC) controls are essential for standardization. This study introduces synthetic protein gels as novel IHC controls, enabling objective quantification and protocol optimization.

Area of Science:

  • Biotechnology
  • Histochemistry
  • Immunohistochemistry

Background:

  • Standardizing immunohistochemistry (IHC) requires reliable positive and negative control samples.
  • Current limitations exist in the availability of suitable tissue or cell line controls for IHC.

Purpose of the Study:

  • To develop a novel method for creating synthetic protein gel-based controls for IHC.
  • To enable objective quantification and standardization of IHC protocols.

Main Methods:

  • Incorporating target antigens (peptides, protein domains, whole proteins) into synthetic protein gels.
  • Utilizing various fixation protocols compatible with the synthetic gels.
  • Creating tissue microarrays (TMAs) with controlled antigen concentrations from the gels.

Main Results:

  • Demonstrated a method to create synthetic protein gels for IHC controls.
  • Showcased compatibility with diverse fixation methods.
  • Enabled the generation of TMAs with tunable antigen levels for calibration.

Conclusions:

  • Synthetic protein gels offer a viable solution for IHC control standardization.
  • This method allows for objective quantification and calibration of IHC staining.
  • Facilitates optimization and inter-laboratory standardization of IHC protocols.

Keywords:
BCL2LODMYCepitopeformaldehydelimit of detectionzinc

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