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Updated: Jan 26, 2026

Preparation and Immunostaining of Myelinating Organotypic Cerebellar Slice Cultures
09:41

Preparation and Immunostaining of Myelinating Organotypic Cerebellar Slice Cultures

Published on: March 20, 2019

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Preparation and Immunostaining of Myelinating Organotypic Cerebellar Slice Cultures

Melina Thetiot1, Rémi Ronzano1, Marie-Stéphane Aigrot1

  • 1Sorbonne Université, Inserm, CNRS, Institut du Cerveau et de la Moelle épinière, ICM-GH Pitié-Salpétrière.

Journal of Visualized Experiments : Jove
|April 9, 2019
PubMed

Insights

This study introduces an ex vivo model using mouse cerebellar slices to visualize myelin, a crucial component of the nervous system. This model aids in studying myelin alterations in neurological diseases.

Area of Science:

  • Neuroscience
  • Cell Biology
  • Histology

Background:

  • Myelin is a vital membrane in the nervous system, produced by glial cells, essential for rapid nerve impulse transmission.
  • Alterations in myelin are implicated in various neurological disorders, leading to impaired function.

Purpose of the Study:

  • To describe a novel ex vivo model for studying myelin.
  • To enable visualization of myelin in a controlled experimental setting.

Main Methods:

  • Utilizing organotypic cerebellar slices from mice.
  • Maintaining slices in culture for extended periods (several weeks).
  • Employing labeling techniques for myelin visualization.

Main Results:

  • Successful establishment and maintenance of ex vivo cerebellar slice cultures.
  • Demonstrated feasibility of myelin labeling and visualization within the cultured slices.

Conclusions:

  • The described ex vivo model provides a valuable platform for investigating myelin structure and function.
  • This model can be instrumental in understanding myelin's role in neurological diseases.

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