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Classification of the Immune Composition in the Tumor Infiltrate

Davide Brusa1, Jean-Luc Balligand2

  • 1Flow Cytometry Platform, Institute of Experimental and Clinical Research (IREC), Université Catholique de Louvain (UCL), Brussels, Belgium. davide.brusa@uclouvain.be.

Methods in Molecular Biology (Clifton, N.J.)
|April 28, 2019
PubMed

Insights

Flow cytometry effectively analyzes immune cells in tumor infiltrates. This technique allows for thorough characterization and isolation of specific immune cell subtypes using single-cell sorting.

Area of Science:

  • Immunology
  • Cell Biology
  • Biotechnology

Background:

  • Flow cytometry is a powerful technique for analyzing cell populations.
  • Characterizing immune cells in tumor microenvironments is crucial for understanding cancer biology.
  • Existing methods may have limitations in isolating specific immune cell subtypes from complex tissues.

Purpose of the Study:

  • To describe a detailed flow cytometry technique for immune cell characterization.
  • To enable thorough analysis of immune cells within tumor infiltrates.
  • To facilitate the isolation of specific immune cell subtypes from tumor samples.

Main Methods:

  • Utilizing flow cytometry with specific antibody panels for surface and intracytoplasmic antigens.
  • Applying single-cell sorting for precise isolation of desired immune cell populations.
  • Detailed protocol for sample preparation from tumor infiltrates.

Main Results:

  • Successful comprehensive characterization of diverse immune cell subtypes.
  • Demonstrated efficacy of single-cell sorting for isolating specific immune cells.
  • High-resolution analysis of immune cell populations within the tumor microenvironment.

Conclusions:

  • The described flow cytometry method provides robust immune cell characterization.
  • Single-cell sorting is a valuable tool for isolating specific immune cells from tumor infiltrates.
  • This technique enhances the study of tumor immunology and facilitates further research.

Keywords:
FACS analysisFlow cytometryImmune infiltrateImmune systemSingle-cell sortingTumor immunology

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