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Updated: Jan 25, 2026

Preparation of Formalin-fixed Paraffin-embedded Tissue Cores for both RNA and DNA Extraction
08:30

Preparation of Formalin-fixed Paraffin-embedded Tissue Cores for both RNA and DNA Extraction

Published on: August 21, 2016

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Immunofluorescence Labeling of Nuclear Receptor Expression in Formalin-Fixed, Paraffin-Embedded Tissue

Elizabeth D Lightbody1,2, Christopher J B Nicol3,4,5

  • 1Department of Pathology and Molecular Medicine, Queen's University, Kingston, ON, Canada.

Methods in Molecular Biology (Clifton, N.J.)
|May 2, 2019
PubMed

Insights

Immunofluorescent staining (IF) visualizes protein expression in tissues using labeled antibodies. This optimized protocol efficiently detects multiple proteins in FFPE samples, aiding in vivo study analysis.

Area of Science:

  • Biotechnology
  • Molecular Biology
  • Histology

Background:

  • Immunofluorescent staining (IF) is crucial for visualizing protein expression in tissue samples.
  • Optimized IF methods allow for multiplexed protein detection within single tissue sections.
  • Formalin-fixed paraffin-embedded (FFPE) tissues are commonly used in research.

Purpose of the Study:

  • To describe an optimized immunofluorescent staining protocol.
  • To enable simultaneous visualization of multiple proteins in FFPE tissues.
  • To facilitate the analysis of nuclear receptor expression in conditional knockout studies.

Main Methods:

  • Utilizing antigen-antibody complexes labeled with fluorochromes.
  • Applying an optimized protocol for immunofluorescence.
  • Processing both mouse and human FFPE tissue samples.
  • Examining protein expression in experimental samples from in vivo studies.

Main Results:

  • Successful visualization of protein expression within tissue sections.
  • Demonstrated ability to detect multiple proteins simultaneously.
  • Routine application of the protocol for nuclear receptor analysis.

Conclusions:

  • The described optimized immunofluorescence protocol is effective for multiplexed protein detection in FFPE tissues.
  • This method is valuable for analyzing protein expression, particularly nuclear receptors, in complex experimental models.
  • The protocol supports robust data acquisition in molecular and histological studies.

Keywords:
FFPEImmunofluorescent labelingNuclear receptor

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