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Updated: Jan 24, 2026

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Effects of blood sample handling procedures on measurable interleukin 6 in plasma and serum

Yan Gong1,2, Shaocong Liang3, Lei Zeng3

  • 1Department of Coloproctology, Zhujiang Hospital, Southern Medical University, GuangZhou, China.

Journal of Clinical Laboratory Analysis
|May 28, 2019
PubMed

Insights

Proper blood sample handling is crucial for accurate Interleukin-6 (IL-6) measurement. Immediate centrifugation and separation of samples ensure IL-6 stability, preventing false results due to storage temperature and time.

Area of Science:

  • Clinical Chemistry
  • Biomarker Analysis
  • Pre-analytical Variables

Background:

  • Interleukin-6 (IL-6) is a vital biomarker for disease diagnosis, therapy, and prognosis.
  • Pre-analytical factors can significantly impact the accuracy of IL-6 measurements.
  • Standardized procedures are needed to ensure reliable IL-6 results.

Purpose of the Study:

  • To investigate the impact of various blood sample handling procedures on Interleukin-6 (IL-6) levels.
  • To identify optimal pre-analytical conditions for stable IL-6 measurements.

Main Methods:

  • Collected EDTA plasma and serum from 45 healthy individuals.
  • Implemented different centrifugation timings, storage temperatures, and durations.
  • Analyzed trends in IL-6 levels under varied handling conditions.

Main Results:

  • Plasma IL-6 levels were higher than serum at baseline, with good correlation.
  • Unseparated EDTA plasma remained stable over time.
  • Unseparated serum IL-6 increased with higher storage temperatures and longer durations.
  • Immediately centrifuged and separated samples demonstrated stability regardless of storage conditions.

Conclusions:

  • Sample type, centrifugation timing, storage temperature, and time significantly influence IL-6 levels.
  • Implementing standardized blood sample handling is essential for accurate and stable IL-6 quantification.
  • Immediate sample processing ensures reliable IL-6 biomarker integrity.
Abstract

Keywords:
centrifugal timinginterleukin-6sample typesstorage timetemperature

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