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Isolation and Characterization of Lymphocytes from Human Mucosal Biopsies

Nadine E G Upton1,2, Thomas S Hayday3, Louisa K James4

  • 1Asthma and Allergy, Randall Division of Cell and Molecular Biophysics, King's College London, London, UK.

Methods in Molecular Biology (Clifton, N.J.)
|June 10, 2019
PubMed

Insights

This study presents an advanced method for isolating viable lymphocytes from human mucosal biopsies, crucial for understanding allergic diseases. The technique ensures maximum utilization of limited samples for detailed cell analysis.

Area of Science:

  • Immunology
  • Cell Biology
  • Allergy Research

Background:

  • The local mucosal environment significantly influences allergic disease initiation and progression.
  • Analyzing tissue-resident lymphocyte subsets requires viable cells from mucosal samples.
  • Current methods may not fully optimize the use of limited clinical biopsies.

Purpose of the Study:

  • To describe an advanced method for dissociating lymphocytes from human mucosal biopsies.
  • To enable comprehensive downstream analysis of tissue-resident lymphocytes.
  • To maximize the utility of limited clinical samples for immunological research.

Main Methods:

  • Gentle mechanical disruption of mucosal tissue.
  • Enzymatic incubation with proteolytic enzymes.
  • Generation of single-cell suspensions from nasal and bronchial biopsies.

Main Results:

  • The described method effectively dissociates lymphocytes from mucosal tissues.
  • Obtained single-cell suspensions are viable for downstream applications.
  • The technique is suitable for analyzing tissue lymphocytes via flow cytometry.

Conclusions:

  • This advanced dissociation method enhances the analysis of mucosal lymphocytes.
  • It provides a robust approach for studying allergic diseases at the cellular level.
  • The method optimizes the use of limited clinical samples for immunological investigations.

Keywords:
BiopsyCell isolationFlow cytometryLungLymphocytesMucosaTissue dissociationTissue-resident lymphocytes

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