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Optimization, Design and Avoiding Pitfalls in Manual Multiplex Fluorescent Immunohistochemistry
09:15

Optimization, Design and Avoiding Pitfalls in Manual Multiplex Fluorescent Immunohistochemistry

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Avoiding pitfalls in diagnostic immunohistochemistry-important technical aspects that every pathologist should know

Rodney T Miller1

  • 11355 River Bend Drive, Dallas, TX 75247-4915, United States.

Seminars in Diagnostic Pathology
|June 23, 2019
PubMed

Insights

This review highlights technical challenges in diagnostic immunohistochemistry (IHC), offering solutions for pathologists. It emphasizes proper controls and techniques for difficult specimens to improve diagnostic accuracy.

Area of Science:

  • Pathology
  • Laboratory Medicine
  • Histotechnology

Background:

  • Diagnostic immunohistochemistry (IHC) is crucial for accurate disease diagnosis.
  • Methodological and interpretational challenges can lead to diagnostic errors.
  • Standardization and quality control are essential for reliable IHC results.

Purpose of the Study:

  • To review technical aspects of diagnostic IHC.
  • To address common pitfalls in IHC methodology and interpretation.
  • To provide practical guidance for practicing pathologists.

Main Methods:

  • Review of existing literature and best practices in IHC.
  • Discussion of problematic aspects in IHC methodology.
  • Illustration and description of common artifacts and useful techniques.

Main Results:

  • Identification of critical areas for IHC quality improvement.
  • Emphasis on the importance of appropriate controls, including multi-tissue controls.
  • Guidance on optimizing IHC for challenging specimens like cytology and decalcified tissues.

Conclusions:

  • Adherence to standardized techniques and proper controls can minimize IHC errors.
  • Advanced techniques can overcome limitations with difficult specimens.
  • This review provides practical strategies to enhance the reliability of diagnostic IHC.

Keywords:
ImmunohistochemistryMethodologyPitfallsQuality control

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