Related Experiment Video
Updated: Jan 20, 2026
Effect of an Immunomodulatory Pharmacological Agent on the Interaction Between Dendritic Cells and CD4+ T Cells
CD5 on dendritic cells regulates CD4+ and CD8+ T cell activation and induction of immune responses
Hui Li1, Erica Burgueño-Bucio2, Shin Xu1
1Department of Dermatology, University of Alabama at Birmingham. Birmingham, Alabama, United States of America.
Insights
The immune system uses CD5 on dendritic cells (DCs) to regulate T cell responses. Removing CD5 from DCs boosts their ability to activate T cells and fight tumors.
Area of Science:
- Immunology
- Cell Biology
Background:
- CD5 is a known regulator of T cell signaling and tolerance.
- Expression of CD5 on human dendritic cells (DCs) is recent data, but its function is poorly understood.
Purpose of the Study:
- To investigate the functional role of CD5 on DCs in modulating immune responses.
- To understand how CD5 expression on DCs impacts T cell activation and cytokine production.
Main Methods:
- CD5 expression was analyzed on murine dendritic cell (DC) subtypes.
- DC subpopulations in CD5 knockout (CD5-/-) and wild-type (CD5+/+) mice were compared.
- Cytokine production (IL-12, IL-2, IFN-gamma) by DCs and T cells was measured.
- In vitro and in vivo T cell activation assays were performed.
- Anti-tumor immunity and contact hypersensitivity responses were assessed in mice.
Main Results:
- CD5 is expressed on CD11c+ DCs in various murine tissues.
- CD5-deficient DCs produced higher levels of IL-12 in response to LPS compared to wild-type DCs.
- CD5-/- DCs enhanced CD4+ and CD8+ T cell activation, leading to increased IL-2 and IFN-gamma production.
- CD5-/- DCs were more potent in inducing anti-tumor immunity and contact hypersensitivity.
- Restoring CD5 expression on CD5-/- DCs reduced IL-12 production and T cell stimulation.
Conclusions:
- CD5 expression on DCs inhibits inflammatory cytokine production.
- CD5 plays a regulatory role in DC activity, impacting T cell stimulation and immune responses.
- This study uncovers a novel regulatory function for CD5 on DCs, offering new insights into DC biology.
Abstract:
The role of CD5 as a regulator of T cell signaling and tolerance is well recognized. Recent data show expression of CD5 on different subtypes of human dendritic cells, however its functional relevance in modulating DC mediated responses remains poorly understood. In this study, we show CD5 is expressed on CD11c+ DC from murine thymus, lymph node, spleen, skin and lung. Although the development of DC subpopulations in CD5-/- mice was normal, CD5-deficient DC produced significantly higher levels of IL-12 than wild type DC in response to LPS. CD5-/- DC, in comparison to CD5+/+ DC, enhanced the activation of CD4+ and CD8+ T cells in vitro and in vivo and induced significantly higher production of IL-2 and IFN-gamma by T cells. Consequently, CD5-/- DC were significantly more potent than wild type DC in the induction of anti-tumor immunity and contact hypersensitivity responses in mice. Restoration of CD5 expression in CD5-/- DC reduced IL-12 production and inhibited their capacity to stimulate T cells. Collectively, these data demonstrate that the specific expression of CD5 on DC inhibits the production of inflammatory cytokines and has a regulatory effect on their activity to stimulate T cells and induce immune responses. This study reveals a previously unrecognized regulatory role for CD5 on DC and provides novel insights into mechanisms for DC biology in immune responses.
More Related Videos
Related Concept Videos
02:40Effect of an Immunomodulatory Pharmacological Agent on the Interaction Between Dendritic Cells and CD4+ T Cells
10:57Cell Cycle Analysis: Assessing CD4 and CD8 T Cell Proliferation After Stimulation Using CFSE Staining and Flow Cytometry
1 Unit for Lymphpoiesis, Department of Immunology, Pasteur Institute, Paris, France
2 INSERM U1223, Paris, France
3 Université Paris Diderot, Sorbonne Paris Cité, Cellule Pasteur, Paris, France
4 Flow Cytometry Platfrom, Cytometry and Biomarkers UtechS, Center for Translational Science, Pasteur Institute, Paris, France
The cell cycle is a universal process of life. During the cell cycle, a cell...
03:35Flow Cytometry-Based Analysis of Dendritic Cell Activation Using Immune Complexes
08:39An In Vivo Mouse Model to Measure Naïve CD4 T Cell Activation, Proliferation and Th1 Differentiation Induced by Bone Marrow-derived Dendritic Cells
16:04A Tetracycline-regulated Cell Line Produces High-titer Lentiviral Vectors that Specifically Target Dendritic Cells
09:55Isolation and Characterization of the Immune Cells from Micro-dissected Mouse Choroid Plexuses