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[Immunochromatographic test for detection of fecal occult blood.]

A V Nikitina1, Y A Akinshina1, N E Nishchakova1

  • 1CJSC "EKOlab", 142530, Elektrogorsk, Moscow region.

Klinicheskaia Laboratornaia Diagnostika
|October 15, 2019
PubMed

Insights

This study developed an immunochromatographic test for fecal occult blood detection. The test accurately identifies hemoglobin in stool, aiding in colorectal cancer screening.

Area of Science:

  • Clinical diagnostics
  • Biochemistry
  • Medical technology

Background:

  • Colorectal cancer screening relies on early detection methods.
  • Fecal occult blood testing is a key component of colorectal cancer screening.
  • Immunochromatographic tests offer rapid diagnostic capabilities.

Purpose of the Study:

  • To develop and evaluate an immunochromatographic test for fecal occult blood (hemoglobin) detection.
  • To assess the diagnostic characteristics, including sensitivity and specificity, of the developed test.
  • To determine the utility of the immunochromatographic test in primary colorectal cancer screening.

Main Methods:

  • Validation using blank and standardized hemoglobin samples at various concentrations.
  • Assessment of test performance against known hemoglobin levels.
  • Evaluation of diagnostic sensitivity and specificity.

Main Results:

  • The immunochromatographic test detected hemoglobin at concentrations above 5 μg/g feces with 97.6% sensitivity.
  • The test demonstrated 100% specificity in identifying fecal occult blood.
  • The developed test system proved effective in detecting low concentrations of hemoglobin.

Conclusions:

  • The immunochromatographic fecal occult blood test is a reliable tool for rapid screening.
  • The test's high sensitivity and specificity support its use in primary colorectal cancer screening.
  • This assay offers a valuable method for early detection of gastrointestinal bleeding indicative of colorectal cancer.

Keywords:
FOB-testfecal occult bloodimmunochromatographic analysisscreening

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