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CD Maps-Dynamic Profiling of CD1-CD100 Surface Expression on Human Leukocyte and Lymphocyte Subsets
Tomas Kalina1,2, Karel Fišer1, Martin Pérez-Andrés3,4
1CLIP - Childhood Leukaemia Investigation Prague, Department of Paediatric Haematology and Oncology, Charles University, Prague, Czechia.
Insights
The CD Maps study comprehensively mapped 110 CD molecules across 47 human immune cell subsets, creating an interactive resource to advance leukocyte biology and biomarker discovery.
Area of Science:
- Immunology
- Cell Biology
- Biotechnology
Background:
- Cluster of Differentiation (CD) molecules are crucial for immune cell function and identification.
- Standardized mapping of CD molecule expression is needed for reproducible research.
Purpose of the Study:
- To map the expression of 110 CD molecules (CD1-CD100) on 47 immune cell subsets.
- To develop an interactive web resource for visualizing and analyzing CD molecule expression data.
- To identify differentially expressed proteins and dynamic marker profiles in immune cell subsets.
Main Methods:
- Utilized an eight-color standardized EuroFlow approach for high-dimensional analysis.
- Quantified CD molecule expression using median antibody binding capacities (ABCs) and percentage of positivity.
- Integrated data into an interactive CD Maps web resource.
Main Results:
- Generated a comprehensive dataset of CD molecule expression across diverse immune cell populations from blood, thymus, and tonsil.
- Identified differential protein expression patterns among granulocyte, monocyte, and dendritic cell subsets.
- Profiled dynamic marker expression during thymocyte differentiation, T-cell maturation, and B-cell subset clustering.
Conclusions:
- The CD Maps resource provides a benchmark for antibody reactivities, enhancing flow cytometry reproducibility.
- Offers a detailed view of the human immune cell surfaceome, aiding understanding of leukocyte biology.
- Facilitates the discovery of novel biomarkers and therapeutic targets for immunological and hematological diseases.
Abstract:
CD molecules are surface molecules expressed on cells of the immune system that play key roles in immune cell-cell communication and sensing the microenvironment. These molecules are essential markers for the identification and isolation of leukocytes and lymphocyte subsets. Here, we present the results of the first phase of the CD Maps study, mapping the expression of CD1-CD100 (n = 110) on 47 immune cell subsets from blood, thymus, and tonsil using an eight-color standardized EuroFlow approach and quantification of expression. The resulting dataset included median antibody binding capacities (ABCs) and percentage of positivity for all markers on all subsets and was developed into an interactive CD Maps web resource. Using the resource, we examined differentially expressed proteins between granulocyte, monocyte, and dendritic cell subsets, and profiled dynamic expression of markers during thymocyte differentiation, T-cell maturation, and between functionally distinct B-cell subset clusters. The CD Maps resource will serve as a benchmark of antibody reactivities ensuring improved reproducibility of flow cytometry-based research. Moreover, it will provide a full picture of the surfaceome of human immune cells and serves as a useful platform to increase our understanding of leukocyte biology, as well as to facilitate the identification of new biomarkers and therapeutic targets of immunological and hematological diseases.
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