Related Experiment Video

Updated: Dec 21, 2025

Static Adhesion Assay for the Study of Integrin Activation in T Lymphocytes
09:14

Static Adhesion Assay for the Study of Integrin Activation in T Lymphocytes

Published on: June 13, 2014

16.6K

Tetraspanin CD53 Promotes Lymphocyte Recirculation by Stabilizing L-Selectin Surface Expression

Maria C Demaria1, Louisa Yeung2, Rens Peeters3

  • 1Department of Immunology and Pathology, Monash University, Alfred Research Alliance, Melbourne, VIC 3004, Australia.

Iscience
|May 20, 2020
PubMed

Insights

The tetraspanin CD53 is crucial for lymphocyte recirculation, enabling immune cells to travel to lymph nodes. Its absence impairs immune responses by affecting cell trafficking and L-selectin stability.

Area of Science:

  • Immunology
  • Cell Biology

Background:

  • Tetraspanins are cell surface proteins involved in immune cell function.
  • The specific role of CD53, a leukocyte-restricted tetraspanin, in immune cell processes remains largely unknown.

Purpose of the Study:

  • To investigate the function of CD53 in lymphocyte recirculation and immune responses.
  • To elucidate the molecular mechanisms by which CD53 influences lymphocyte homing.

Main Methods:

  • Analysis of lymph node cellularity and lymphocyte populations in Cd53 knockout (Cd53-/-) mice.
  • Assessment of L-selectin expression and stability on lymphocytes from Cd53-/- and wild-type mice.
  • Investigation of CD53's role in regulating L-selectin shedding in both mouse and human lymphocytes.

Main Results:

  • Cd53-/- mice exhibited smaller lymph nodes with significantly reduced B and T cell numbers.
  • Cd53-/- lymphocytes showed impaired homing to lymph nodes, linked to L-selectin deficiency or instability.
  • CD53 was found to stabilize L-selectin surface expression and inhibit its shedding through both ADAM17-dependent and -independent pathways.
  • Disrupted lymphocyte recirculation in Cd53-/- mice resulted in compromised immune responses.

Conclusions:

  • CD53 plays an essential role in regulating lymphocyte recirculation by stabilizing L-selectin.
  • The findings highlight CD53 as a key regulator of lymphocyte trafficking and overall immune function.

Keywords:
Immune ResponseImmunologyMolecular Mechanism of BehaviorSpecialized Functions of Cells

More Related Videos

Isolation of Murine Lymph Node Stromal Cells05:47

Isolation of Murine Lymph Node Stromal Cells

Published on: August 19, 2014

32.0K
Isolation of Human Lymphatic Endothelial Cells by Multi-parameter Fluorescence-activated Cell Sorting07:36

Isolation of Human Lymphatic Endothelial Cells by Multi-parameter Fluorescence-activated Cell Sorting

Published on: May 1, 2015

14.8K

Related Concept Videos

Selectins01:25

Selectins

Cell adhesion is  an essential aspect of multicellularity. While stable cell interactions usually occur between cells of the same type, transient cell interactions occur between cells of different tissue types, such as between neutrophils and endothelial cells. Selectins are one class of cell adhesion molecules (CAMs) that bind carbohydrate ligands to form transient cell adhesion. They are rod-like proteins with a long extracellular part of variable length ending with the lectin domain,...
3.9K
T Cell Activation and Clonal Selection01:22

T Cell Activation and Clonal Selection

T cells are integral to our adaptive immune system, recognizing and effectively responding to foreign antigens. T cell activation and clonal selection are pivotal in orchestrating this immune response. This article elucidates these mechanisms, detailing the roles of cluster of differentiation (CD) markers, major histocompatibility complex (MHC) molecules, costimulatory signals, and the process of clonal selection.
Naive T cells that have not yet encountered an antigen express two primary CD...
14.4K
Role of Ephrin-Eph Signalling in Intestinal Stem Cell Renewal01:22

Role of Ephrin-Eph Signalling in Intestinal Stem Cell Renewal

Erythropoietin-producing hepatocellular carcinoma receptor (Eph) and its ligand, Eph receptor-interacting protein (Ephrin) were first discovered in the human carcinoma cell line, hence the name. Ephrin-Eph interaction guides cells to reach their appropriate location in adult tissues. They also play an essential role in the immune system by helping in immune cell migration, adhesion, and activation. Based on their structure and function, Eph is divided into two classes — EphA and EphB.
2.5K
Intracellular Signaling Affects Focal Adhesions01:17

Intracellular Signaling Affects Focal Adhesions

Integrins act both as extracellular input receivers and as intracellular processing activators. As their name suggests, integrins are entirely integrated into the membrane structure. Their hydrophobic membrane-spanning regions interact with the phospholipid bilayer's hydrophobic region. These membrane receptors provide extracellular attachment sites for effectors like hormones and growth factors. They activate intracellular response cascades when their effectors are bound and active.
Some...
3.4K