A novel oriented immunosensor based on AuNPs-thionine-CMWCNTs and staphylococcal protein A for interleukin-6 analysis...

Area of Science:

• Biomedical Engineering
• Biosensor Technology
• Immunology

Background:

• Interleukin-6 (IL-6) is vital for immune homeostasis, hematopoiesis, and metabolism, making it crucial for disease diagnosis and prognosis.
• Current methods for IL-6 detection may face limitations in sensitivity or specificity.
• Developing advanced biosensors is essential for accurate and efficient monitoring of disease biomarkers.

Purpose of the Study:

• To develop and characterize a novel immunosensor for sensitive and selective detection of Interleukin-6 (IL-6).
• To evaluate the performance of the immunosensor in biological samples, including serum and tissue extracts.
• To provide a new tool for clinical monitoring and research on IL-6 related conditions.

Main Methods:

• Fabrication of a highly conductive substrate using gold nanoparticles (Au NPs), thionine (THI), and carboxylated multi-walled carbon nanotubes (CMWCNTs).
• Utilized Staphylococcal protein A (SPA) for oriented immobilization of antibodies, minimizing steric hindrance.
• Electrochemical detection method to quantify IL-6 concentration based on the sensor's response.

Main Results:

• The developed immunosensor demonstrated a low limit of detection (LOD) of 2.87 pg/mL for IL-6.
• Achieved a wide linear detection range from 0.01 ng/mL to 800 ng/mL in serum samples.
• Successfully quantified IL-6 levels in rat serum and various tissue homogenates from myocardial infarction models.

Conclusions:

• The Au NPs-THI-CMWCNTs based immunosensor offers a highly sensitive and reliable platform for IL-6 detection.
• The sensor's ability to detect IL-6 in complex biological matrices like serum and tissue homogenates highlights its clinical potential.
• This novel biosensor provides a valuable approach for studying inflammation and disease progression, particularly in animal models.