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Sandwich-Based Antibody Arrays for Protein Detection

Ying Qing Mao1, Brianne Petritis2, Ruo-Pan Huang2,3,4,5

  • 1RayBiotech Life, Peachtree Corners, GA, USA. mike@raybiotech.com.

Methods in Molecular Biology (Clifton, N.J.)
|November 25, 2020
PubMed

Insights

This study introduces a rapid, 1-day method for processing samples using quantitative sandwich-based antibody arrays. This fluorescence-based technique allows for simultaneous detection of multiple proteins, offering a cost-effective alternative to traditional single-protein assays.

Area of Science:

  • Biochemistry
  • Immunotechnology
  • Proteomics

Background:

  • Sandwich-based antibody arrays allow for multiplexed protein detection.
  • These arrays offer a time- and cost-effective alternative to single-plex assays.
  • The "sandwich" technique involves a capture antibody and a detection antibody.

Purpose of the Study:

  • To describe a streamlined 1-day procedure for sample processing.
  • To utilize quantitative sandwich-based antibody arrays on a glass substrate.
  • To enable fluorescence-based detection of multiple proteins.

Main Methods:

  • Development of a 1-day protocol for sample processing.
  • Application of quantitative sandwich-based antibody arrays.
  • Utilizing fluorescence for protein detection on a glass substrate.
  • Processing of biological samples like serum and cell lysates.

Main Results:

  • Successful implementation of a 1-day procedure.
  • Demonstration of multiplexed protein detection capabilities.
  • Quantitative analysis of proteins in serum and cell lysates.

Conclusions:

  • The described method provides an efficient and cost-effective approach for multiplexed protein analysis.
  • This 1-day protocol facilitates rapid biomarker discovery and validation.
  • The fluorescence-based antibody array platform is suitable for various biological samples.

Keywords:
AntibodiesAntibody arrayArrayFluorescenceImmunoassayQuantitative arraySandwich immunoassay

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