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Updated: Nov 24, 2025
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Published on: June 25, 2021
An optimized protocol for combined fluorescent lectin/immunohistochemistry to characterize tissue-specific glycan
Ana Lúcia Rebelo1, Paolo Contessotto1, Kieran Joyce1,2
1CÚRAM SFI Research Centre for Medical Devices, National University of Ireland Galway, Galway H92 W2TY, Ireland.
Insights
This study details a lectin histochemistry protocol for analyzing tissue glycan distribution. It combines lectin binding specificity with stereological methods for precise quantification in various tissues.
Area of Science:
- Histochemistry
- Glycobiology
- Immunohistochemistry
Background:
- Lectin histochemistry combined with immunohistochemistry is crucial for understanding glycan distribution.
- Characterizing glycan spatial distribution aids in correlating them with specific cell types or antigens.
- Existing methods require detailed protocols for diverse tissue applications.
Purpose of the Study:
- To present a detailed protocol for lectin histochemical analysis of tissues.
- To highlight the application of monosaccharide motif specificity in lectin binding.
- To incorporate stereological methods for quantitative analysis of tissue samples.
Main Methods:
- Lectin histochemistry utilizing monosaccharide motif specificity.
- Immunohistochemistry for antigen detection.
- Stereological methods for tissue quantification.
Main Results:
- The protocol enables detailed characterization of glycan spatial distribution in various tissues.
- Monosaccharide specificity of lectins allows for precise glycan identification.
- Stereological methods provide quantitative data on lectin binding and cell populations.
Conclusions:
- This protocol offers a robust method for analyzing tissue glycans.
- The combination of lectin histochemistry and stereology enhances tissue analysis.
- The described techniques are valuable for research in glycobiology and cell biology.
Abstract:
Lectin histochemical analysis of tissues combined with immunohistochemistry is a valuable tool to characterize and correlate the spatial distribution of glycans with the presence of specific cell types or antigens of interest. The current protocol describes the application of monosaccharide motif specificity of lectin binding to glycan residues to different tissue types. In addition, we describe stereological methods to provide further quantification of the analyzed tissues. For complete details on the use and execution of this protocol, please refer to Mohd Isa et al. (2018), Contessotto et al. (2020), and Samal et al. (2020).
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