Related Experiment Video

Updated: Nov 17, 2025

An Efficient and High Yield Method for Isolation of Mouse Dendritic Cell Subsets
09:09

An Efficient and High Yield Method for Isolation of Mouse Dendritic Cell Subsets

Published on: April 18, 2016

15.7K

Soluble CD83 Regulates Dendritic Cell-T Cell Immunological Synapse Formation by Disrupting Rab1a-Mediated F-Actin

Wei Lin1, Shuping Zhou1, Meng Feng1

  • 1Institute of Basic Medicine, Shandong Provincial Hospital Affiliated to Shandong First Medical University, Shandong First Medical University & Shandong Academy of Medical Science, Jinan, China.

Frontiers in Cell and Developmental Biology
|February 15, 2021
PubMed

Insights

Soluble CD83 (sCD83) inhibits T cell activation by disrupting dendritic cell (DC) cytoskeletal rearrangements and DC-T cell contact. This pathway offers potential for new immunosuppressive therapies for autoimmune diseases.

Area of Science:

  • Immunology
  • Cell Biology
  • Autoimmune Disease Research

Background:

  • Dendritic cell-T cell (DC-T) interactions are crucial for T cell activation and immune responses.
  • Cytoskeletal regulation in DCs significantly influences DC-T cell contact and subsequent T cell activation.
  • Inhibitory factors affecting DC cytoskeletal regulation are not well understood.

Purpose of the Study:

  • To investigate the role of soluble CD83 (sCD83) in modulating DC-T cell interactions and T cell activation.
  • To elucidate the molecular mechanisms by which sCD83 affects DC cytoskeletal dynamics and synapse formation.
  • To evaluate the therapeutic potential of sCD83 in an animal model of autoimmune disease.

Main Methods:

  • Treatment of DCs with sCD83.
  • Assessment of DC-T cell contact, synapse formation, and T cell activation.
  • Analysis of F-actin rearrangements, MHC-II expression and localization, and Rab1a GTP-binding activity.
  • Evaluation of sCD83-treated DCs in a mouse model of experimental autoimmune uveitis.

Main Results:

  • sCD83 inhibited T cell activation by reducing DC-T cell contact and synapse formation.
  • sCD83 disrupted F-actin rearrangements in DCs, altering MHC-II expression/localization and synapse formation.
  • sCD83 decreased Rab1a GTP-binding activity, impacting LRRK2 colocalization and F-actin rearrangements.
  • sCD83-treated DCs ameliorated experimental autoimmune uveitis symptoms in mice.

Conclusions:

  • sCD83 negatively regulates DC-T cell contact and T cell activation through cytoskeletal disruption.
  • A novel signaling pathway involving sCD83, Rab1a, LRRK2, and F-actin impacts DC function.
  • sCD83 demonstrates therapeutic potential for treating autoimmune diseases like uveitis.

Keywords:
DC-T contactRab1Aautoimmune uveitisimmunological synapsesCD83

More Related Videos

Assessment of the Synaptic Interface of Primary Human T Cells from Peripheral Blood and Lymphoid Tissue06:27

Assessment of the Synaptic Interface of Primary Human T Cells from Peripheral Blood and Lymphoid Tissue

Published on: July 30, 2018

6.8K
An Endothelial Planar Cell Model for Imaging Immunological Synapse Dynamics09:25

An Endothelial Planar Cell Model for Imaging Immunological Synapse Dynamics

Published on: December 24, 2015

9.2K

Related Concept Videos

T Cell Activation and Clonal Selection01:22

T Cell Activation and Clonal Selection

T cells are integral to our adaptive immune system, recognizing and effectively responding to foreign antigens. T cell activation and clonal selection are pivotal in orchestrating this immune response. This article elucidates these mechanisms, detailing the roles of cluster of differentiation (CD) markers, major histocompatibility complex (MHC) molecules, costimulatory signals, and the process of clonal selection.
Naive T cells that have not yet encountered an antigen express two primary CD...
14.1K
Receptor Downregulation in MVBs01:15

Receptor Downregulation in MVBs

Multivesicular bodies (MVBs) are mature endosomes that sort ubiquitinated proteins and then fuse with lysosomes to degrade the sorted proteins. Epidermal growth factor (EGF) and its receptor (EGFR) form a complex that can be internalized through endocytosis, sorted into an MVB, and later degraded.
The EGFR can initiate signaling pathways that  lead to cell proliferation, migration, and differentiation. Overexpression of EGFR  stimulates cells to proliferate. Excessive  EGFR...
2.5K
Immunoglobulin-like Cell Adhesion Molecules01:31

Immunoglobulin-like Cell Adhesion Molecules

Immunoglobulin-like cell adhesion molecules or Ig-CAMs are a versatile group of cell surface glycoproteins belonging to the immunoglobulin protein superfamily. Ig-CAMs possess the characteristic immunoglobulin protein domains and other domains such as the fibronectin type III domain. The Ig domains are glycosylated to varying degrees in different Ig-CAMs.
Ig-CAMs exhibit either homophilic binding (to other Ig-CAMs) or heterophilic binding (to other ligands such as integrins). While most Ig-CAMs...
3.8K
Rab Cascades01:25

Rab Cascades

Rab GTPases act in a regulated cascade during membrane fusion, helping the lipid bilayers mix. The Rab family of proteins are active when bound to GTP, and inactive when bound to GDP. Hence, they act as guanine nucleotide-dependent molecular switches. Rab-GTP recognizes and binds to long or short-range tethering proteins to capture the target vesicle. These tethers coordinate with SNAREs on the vesicle and the target membrane to assemble the trans SNARE complex that locks the mixing bilayers.
3.2K