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Urinary gonadotropin measurements by enhanced luminometric assays (LIA) for the evaluation of pubertal development

And Demir1, Adem Aydın2, Atilla Büyükgebiz3

  • 1New Children's Hospital, Pediatric Research Center, University of Helsinki, Helsinki University Hospital, Helsinki, Finland.

Journal of Pediatric Endocrinology & Metabolism : JPEM
|April 29, 2021
PubMed

Insights

The enhanced luminometric assay (LIA) effectively measures urinary gonadotropins, providing a reliable alternative for assessing pubertal development and the transition from prepuberty to puberty.

Area of Science:

  • Endocrinology
  • Reproductive Medicine
  • Biochemical Assays

Background:

  • Urinary luteinizing hormone (LH) measurement is a validated method for evaluating pubertal development.
  • Time-resolved immunofluorometric assay (IFMA) is a sensitive technique for LH determination but may not be universally available.
  • Alternative sensitive assays for urinary gonadotropin measurement require evaluation.

Purpose of the Study:

  • To evaluate the enhanced luminometric assay (LIA) as a potential alternative to IFMA for measuring urinary gonadotropins.
  • To assess the utility of LIA in determining pubertal development stages based on urinary LH and FSH levels.

Main Methods:

  • LIA was employed to quantify LH and FSH in serum and urine samples from 100 healthy individuals per Tanner stage and gender.
  • Pubertal development status was determined for all participants.

Main Results:

  • Urinary gonadotropin levels measured by LIA showed strong correlations with Tanner stage for both LH and FSH in girls and boys.
  • LIA detected significant increases in urinary LH from Tanner stage 1-2 in both genders.
  • Urinary FSH and serum LH detected these transitions only in specific genders.

Conclusions:

  • Measurement of urinary gonadotropins using LIA is a viable method for assessing overall pubertal development.
  • LIA can effectively identify the transition from prepuberty to puberty through urinary gonadotropin analysis.
Abstract

Keywords:
IFMALIAluteinizing hormoneprepubertypubertyserumurinary gonadotropinsurine

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