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Detection of monoclonal protein by capillary zone electrophoresis can be challenged by iodinated contrast agent

Clément Capaldo1, Mourad Cheddad El Aouni2, Damien Laurelli1

  • 1Department of Biochemistry and Pharmaco-Toxicology, University Hospital of Brest, Brest, France.

Biochemia Medica
|April 30, 2021
PubMed

Insights

Iodinated contrast media can interfere with capillary zone electrophoresis (CZE) detection of monoclonal immunoglobulins, potentially masking results. Confirmation requires specific immunochemical methods to ensure accurate diagnosis of monoclonal gammopathy.

Area of Science:

  • Clinical Chemistry
  • Protein Analysis
  • Diagnostic Techniques

Background:

  • Monoclonal gammopathy diagnosis relies on detecting monoclonal immunoglobulins.
  • Capillary zone electrophoresis (CZE) is a standard clinical method for protein screening and measurement.
  • Exogenous substances can interfere with UV-based detection methods.

Observation:

  • Iodinated contrast agents absorb UV light at similar wavelengths to peptide bonds.
  • This absorption can interfere with the detection of proteins during CZE.
  • A case study illustrates how contrast media can mask monoclonal immunoglobulins.

Findings:

  • Iodinated contrast media can lead to false-negative results in CZE for monoclonal proteins.
  • Interference occurs due to the UV absorbance properties of iodine-containing compounds.
  • Confirmation of interference is crucial for accurate diagnosis.

Implications:

  • Clinical laboratories must be aware of potential contrast media interference in CZE.
  • Immunofixation, immunosubtraction, or repeat CZE are necessary to confirm interference.
  • Accurate diagnosis of monoclonal gammopathy requires ruling out contrast media interference.

Keywords:
capillary electrophoresiscase reportgammopathyinterferenceiodinated contrast

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