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Dynamics of Macrophages and Polymorphonuclear Leukocytes Milk-Secreted by Buffaloes with Udders Characterized by

Maria Chiara Alterisio1, Paolo Ciaramella1, Jacopo Guccione1

  • 1Department of Veterinary Medicine and Animal Productions, University of Napoli Federico II, 80137 Napoli, Italy.

Veterinary Sciences
|October 22, 2021
PubMed

Insights

Differential somatic cell count in Mediterranean Buffalo milk reveals distinct macrophage and polymorphonuclear leukocyte dynamics during mastitis. These cell changes offer valuable insights into udder health status.

Area of Science:

  • Veterinary Immunology
  • Ruminant Health
  • Dairy Science

Background:

  • Mastitis significantly impacts dairy ruminant health and productivity.
  • Understanding immune cell dynamics in milk is crucial for diagnosing and managing mastitis.
  • Mediterranean Buffaloes (MBs) represent an important dairy livestock population where udder health requires investigation.

Purpose of the Study:

  • To evaluate the dynamics of macrophages and polymorphonuclear leukocytes in milk from Mediterranean Buffaloes with different udder health statuses.
  • To correlate differential somatic cell count (DSCC) with quantitative somatic cell count (QSCC) and clinical findings.
  • To assess the potential of DSCC as a diagnostic tool for udder health in MBs.

Main Methods:

  • Collected 60 quarter-milk samples from MBs, categorized into clinical mastitis (CM), subclinical mastitis (SCM), intramammary infection (IMI), and healthy controls (n=20 each).
  • Assessed health status using clinical examination, QSCC, and bacteriological milk culture.
  • Performed DSCC to determine the percentage of macrophages and polymorphonuclear leukocytes in milk samples.

Main Results:

  • Mean macrophage percentages differed significantly in CM and SCM quarters compared to healthy ones.
  • Mean polymorphonuclear leukocyte percentages showed significant differences between CM, SCM, and IMI quarters versus healthy quarters.
  • QSCC exhibited a weak negative correlation with macrophages (r = -0.388) and a moderate positive correlation with polymorphonuclear leukocytes (r = 0.477).
  • Macrophages and polymorphonuclear leukocytes displayed a weak negative correlation (r = -0.247).

Conclusions:

  • Milk immune cell dynamics, specifically macrophages and polymorphonuclear leukocytes, provide beneficial information on the clinical status of buffalo udders.
  • DSCC shows potential as a valuable tool for improving udder health management in Mediterranean Buffaloes.
  • Further research into DSCC applications could enhance diagnostic capabilities for ruminant mastitis.

Keywords:
differential somatic cell counthost-responseinnate immunitymastitiswater buffalo

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