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High-Dimensional Immunophenotyping with 37-Color Panel Using Full-Spectrum Cytometry
Marco A Fernandez1, Hammad Alzayat1, Maria C Jaimes2
1Flow Cytometry Facility, Germans Trias i Pujol Research Institute, Badalona, Spain.
Insights
This study introduces a novel 37-color flow cytometry protocol for detailed immune cell analysis. It effectively identifies over 45 cell populations in COVID-19 patients, advancing immunophenotyping capabilities.
Area of Science:
- Immunology
- Cell Biology
- Biotechnology
Background:
- Comprehensive immunophenotyping is crucial for understanding immune system components.
- Conventional flow cytometry has limitations in analyzing numerous cell subsets simultaneously.
- Novel full-spectrum technology offers enhanced data acquisition per sample.
Purpose of the Study:
- To describe an effective and practical protocol for deep and broad immunophenotyping.
- To leverage full-spectrum technology for expanded biological insights.
- To present a 37-color flow cytometry protocol for analyzing SARS-CoV-2 infected patients' blood samples.
Main Methods:
- Development and application of a 37-color flow cytometry panel.
- Utilizing full-spectrum technology for maximal dye information.
- Analysis of whole blood samples from SARS-CoV-2 infected patients.
Main Results:
- Identification of more than 45 distinct immune cell populations.
- Demonstration of the protocol's effectiveness in complex biological samples.
- Successful application in the context of SARS-CoV-2 infection.
Conclusions:
- The 37-color protocol enables comprehensive immunophenotyping.
- Full-spectrum technology significantly enhances data output in flow cytometry.
- This method provides expanded knowledge of immune responses in SARS-CoV-2 patients.
Abstract:
A comprehensive study of the cellular components of the immune system demands both deep and broad immunophenotyping of numerous cell subsets in an effective and practical way. Novel full-spectrum technology reveals the complete emission spectrum of each dye maximizing the amount of information that can be obtained on a single sample regarding conventional flow cytometry and provide an expanded knowledge of biological processes. In this chapter, we describe a 37-color protocol that allows to identify more than 45 different cell populations on whole blood samples of SARS-CoV-2-infected patients.
