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Guidelines for the use of flow cytometry and cell sorting in immunological studies (third edition)
Andrea Cossarizza1, Hyun-Dong Chang2,3, Andreas Radbruch2
1Department of Medical and Surgical Sciences for Children & Adults, University of Modena and Reggio Emilia, Modena, Italy.
Insights
This updated guide details flow cytometry methods for immune cell analysis in humans and mice. It covers experimental design, cell phenotypes, and applications in disease research.
Area of Science:
- Immunology
- Biotechnology
Background:
- Flow cytometry is a critical technique for analyzing immune cells.
- Accurate experimental design and data interpretation are essential for reliable results.
Purpose of the Study:
- To provide updated, comprehensive guidelines for performing flow cytometry experiments.
- To detail immune cell phenotypes and functional assays for human and murine models.
- To offer practical insights into clinical sample analysis and common pitfalls.
Main Methods:
- Comprehensive review of flow cytometry techniques.
- Detailed descriptions of immune cell phenotypes and functional assays.
- Inclusion of comparative tables for human and murine cells.
- Case studies of flow cytometry applications in various diseases.
Main Results:
- The guidelines offer detailed information on immune cell subsets.
- Comparative tables highlight human vs. murine cell differences.
- Clinical applications in autoimmune diseases, cancers, and infectious diseases are presented.
Conclusions:
- The third edition serves as an essential, state-of-the-art handbook for researchers.
- It enhances the understanding and application of flow cytometry in basic and clinical research.
- The guidelines aim to improve experimental reproducibility and data quality.
Abstract:
The third edition of Flow Cytometry Guidelines provides the key aspects to consider when performing flow cytometry experiments and includes comprehensive sections describing phenotypes and functional assays of all major human and murine immune cell subsets. Notably, the Guidelines contain helpful tables highlighting phenotypes and key differences between human and murine cells. Another useful feature of this edition is the flow cytometry analysis of clinical samples with examples of flow cytometry applications in the context of autoimmune diseases, cancers as well as acute and chronic infectious diseases. Furthermore, there are sections detailing tips, tricks and pitfalls to avoid. All sections are written and peer-reviewed by leading flow cytometry experts and immunologists, making this edition an essential and state-of-the-art handbook for basic and clinical researchers.
