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Establishment and Clinical Application of a Method for Detecting T Lymphocyte Subsets by Cellular Immunochip
Chen Chen1, Yan-Mei Liu1, Shu-Xia Xuan1
1Department of Laboratory Medicine, The Sixth Affiliated Hospital of Guangzhou Medical University, Qingyuan People's Hospital, Qingyuan, People's Republic of China.
Insights
Cellular immune chip technology accurately detects T lymphocyte subsets (CD3+, CD4+, CD8+) for immune status analysis. This method is fast, simple, and valuable for clinical diagnosis and prognosis in various populations.
Area of Science:
- Immunology
- Clinical Diagnostics
- Biotechnology
Background:
- Peripheral blood T lymphocyte immune phenotyping is crucial for assessing immune status.
- Existing methods like flow cytometry can be time-consuming and complex.
- A need exists for efficient and accurate T lymphocyte subset detection in clinical settings.
Purpose of the Study:
- To establish and validate a cellular immune chip technology for detecting peripheral blood T lymphocyte subsets (CD3+, CD4+, CD8+, CD4/CD8 ratio).
- To analyze the immune status and clinical diagnostic value of this method in diverse populations within the Qingyuan area.
- To compare the performance of cellular immune chip technology against flow cytometry.
Main Methods:
- Cellular immune chip technology was employed to quantify T lymphocyte subsets (CD3+, CD4+, CD8+, CD4/CD8).
- Accuracy and precision were verified by comparing results with flow cytometry.
- Large-scale detection was conducted on 8389 cases to analyze immune function differences across various groups.
Main Results:
- The cellular immune chip method demonstrated comparable accuracy and precision to flow cytometry, offering speed and simplicity for clinical application.
- Statistical differences in T lymphocyte subset counts were observed among individuals undergoing physical examinations, those with inflammation, and cancer patients.
- Variations in T cell counts and CD4/CD8 ratios were noted between different cancer types and diseases.
Conclusions:
- Cellular immune chip technology provides a practical, accurate, and rapid method for detecting T lymphocyte subsets.
- This technology is suitable for immune function monitoring and prognosis evaluation in patients with various diseases.
- The method holds significant potential for widespread clinical application and popularization.
Objective:
To establish and verify the method for detecting the immune phenotype of peripheral blood T lymphocytes by cellular immune chip technology, analyze the immune status, and discuss its clinical diagnostic value of different populations in the Qingyuan area.
Methods:
First, a cellular immune chip was used to detect the number of T lymphocyte subsets CD3+, CD4+, CD8+, and CD4/CD8, followed by evaluating the accuracy and precision through a comparison with flow cytometry. After passing the performance verification, a large-scale detection was performed by a cellular immune chip in 8389 cases. Immunochip technology detects the expression of T lymphocyte subsets and analyzes the differences in cellular immune function among people with physical examination, inflammation, and cancer, as well as different cancer types and in genders.
Results:
The cell immunochip method and flow cytometry method have the same accuracy and precision in detecting specimens, and the former is fast and simple, and is suitable for clinical use; big data analysis is expected to establish a reference range for CD3+, CD4+, and CD8+ T cell counts in Qingyuan. There are statistical differences in CD3+, CD4+, CD8+ T cell counts in physical examination, inflammation and cancer populations; there are also certain differences in CD3+, CD4+, CD8+ T cell counts and CD4/CD8 ratios between different cancer types and different diseases.
Conclusion:
The method of cell immunochip technology to detect T lymphocyte subsets is simple and practical, with accurate results and rapid detection. It can be used for immune function monitoring and treatment prognosis evaluation of people with different diseases, and it is worthy of popularization and application in clinical practice.
