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Updated: Oct 6, 2025

Cryosectioning of Contiguous Regions of a Single Mouse Skeletal Muscle for Gene Expression and Histological Analyses
08:17

Cryosectioning of Contiguous Regions of a Single Mouse Skeletal Muscle for Gene Expression and Histological Analyses

Published on: December 12, 2016

16.0K

Cryosectioning

Mark A Ross1, Lauryn Kohut2, Patricia A Loughran1,2

  • 1University of Pittsburgh, Center for Biologic Imaging, Pittsburgh, Pennsylvania.

Current Protocols
|January 17, 2022
PubMed

Insights

This study details cryosectioning protocols for preparing tissue samples. These methods are essential for various light microscopy techniques like histochemistry and immunofluorescence.

Area of Science:

  • Histology and Microscopy
  • Biotechnology
  • Cell Biology

Background:

  • Accurate tissue preparation is crucial for reliable light microscopy results.
  • Cryosectioning is a key technique for preserving tissue morphology and antigenicity.
  • Established protocols ensure reproducibility in histochemistry and immunohistochemistry.

Purpose of the Study:

  • To provide detailed protocols for cryosectioning tissue samples.
  • To facilitate the use of cryosectioned tissues in histochemistry, enzyme immunohistochemistry, and immunofluorescence.
  • To ensure high-quality sample preparation for advanced light microscopy.

Main Methods:

  • Detailed step-by-step guide for cryosectioning.
  • Sample preparation for cryo-embedding.
  • Sectioning techniques for optimal tissue preservation.

Main Results:

  • Successfully cryosectioned tissue samples suitable for light microscopy.
  • Demonstrated feasibility of using cryosections for histochemistry.
  • Validated cryosectioning for enzyme immunohistochemistry and immunofluorescence.

Conclusions:

  • The presented cryosectioning protocols are effective for preparing tissue samples for diverse light microscopy applications.
  • These methods support high-resolution imaging and accurate analysis in biological research.
  • Standardized cryosectioning enhances the reliability of histochemical and immunofluorescence staining.

Keywords:
cryopreservationcryosectioning and immunofluorescencetissue perfusion-fixation

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