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Flow-Cytometry in the Diagnosis of Diffuse Large B-Cell Lymphoma Based on Stomach Tissue Samples: A Case Report

Tung Tuan Nguyen1, An Thi Vinh Do1, Nga Thi Nguyen2

  • 1Hematology and Blood Transfusion, Bach Mai Hospital, Hanoi, VNM.

Cureus
|February 10, 2022
PubMed

Insights

Multicolor flow cytometry is a novel technique for diagnosing lymphoma. This method successfully identified diffuse large B-cell lymphoma in a patient's stomach tissue biopsy when other methods were ambiguous.

Area of Science:

  • Hematology
  • Oncology
  • Immunology

Background:

  • Morphology and immunohistochemistry are standard diagnostic tools for lymphoma staging and subtyping.
  • These methods analyze lymph node, tissue, and bone marrow biopsies.
  • Challenges arise when immunohistochemical staining results are ambiguous.

Observation:

  • A case report details a patient diagnosed with lymphoma.
  • The diagnosis was made using fresh tissue from a stomach biopsy.
  • Multicolor flow cytometry was employed for immunological marker analysis.

Findings:

  • Multicolor flow cytometry is a novel technique for lymphoma identification.
  • This technology analyzes immunological markers on fresh tissues.
  • Flow cytometry provided a definitive diagnosis in this case.

Implications:

  • Flow cytometry offers a valuable alternative for lymphoma diagnosis, especially in ambiguous cases.
  • This technique aids in characterizing lymphoma subtypes and stages.
  • It enhances diagnostic accuracy for hematological malignancies.

Keywords:
diffuse large b-cell lymphomaflow cytometryfresh tissueimmunohistochemistrylymphoma

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