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Carboxymethyl chitosan assembled piezoelectric biosensor for rapid and label-free quantification of immunoglobulin Y
1Hubei Key Laboratory of Biomass Resource Chemistry and Environmental Biotechnology, Hubei International Scientific and Technological Cooperation Base of Sustainable Resource and Energy, School of Resource and Environmental Science, Wuhan University, Wuhan 430079, China.
Insights
A new piezoelectric biosensor using carboxymethyl chitosan (CMCS) offers a rapid, cost-effective method for quantifying Immunoglobulin Y (IgY). This label-free approach demonstrates high sensitivity and prevents non-specific binding, making it suitable for diagnostics.
Area of Science:
- Biotechnology
- Biosensor Technology
- Analytical Chemistry
Background:
- Immunoglobulin Y (IgY) offers advantages over Immunoglobulin G (IgG) in prophylaxis and diagnostics.
- Accurate quantification of IgY is crucial for its effective application.
- Development of sensitive and efficient IgY detection methods is of significant interest.
Purpose of the Study:
- To develop and demonstrate a novel piezoelectric biosensor for IgY quantification.
- To evaluate carboxymethyl chitosan (CMCS) as an immobilization matrix for antibodies.
- To compare CMCS with carboxymethyl cellulose (CMC) for antibody immobilization efficiency.
Main Methods:
- Fabrication of a piezoelectric biosensor utilizing carboxymethyl chitosan (CMCS) as the immobilization matrix.
- Investigation of CMCS properties (gelation, hydrophilicity) for antibody immobilization.
- Comparative analysis of CMCS versus CMC for antibody immobilization and biosensor performance.
Main Results:
- Established a calibration curve for IgY quantification from 500 ng/mL to 200 μg/mL with a detection limit (LOD) of 270 ng/mL.
- Demonstrated effective prevention of non-specific binding by bovine serum albumin (BSA) and lysozyme.
- Achieved rapid real-time assay times of 15 minutes, including sensor regeneration, with signal readout in 4 minutes.
Conclusions:
- The developed CMCS-based piezoelectric biosensor provides a feasible, rapid, and label-free method for IgY quantification.
- CMCS offers advantages as an immobilization matrix compared to CMC, enhancing biosensor performance.
- The biosensor is suitable for repeated measurements and potential use in disposable devices for IgY detection.
Abstract:
Immunoglobulin Y (IgY) proves advantageous to IgG in prophylaxis and diagnosis. Quantification of IgY is therefore becoming a topic of interest. Here, we demonstrate a piezoelectric biosensor with carboxymethyl chitosan (CMCS) as the immobilization matrix. Gelation and hydrophilic nature of CMCS are favored to form a crosslinked matrix for antibody immobilization, and a comparison was made between carboxymethyl cellulose (CMC) and CMCS to investigate the benefits of such substitution. Calibration from 500 ng/mL to 200 μg/mL was established in buffer with the detection limit (LOD) down to 270 ng/mL, confirming its feasibility. As-prepared biosensor effectively prevents non-specific binding of bovine serum albumin (BSA) and lysozyme. Each real-time assay took 15 min including sensor regeneration, which can be further reduced to 4 min for signal readout only, ready for both repeated measurements after regeneration and disposable devices. Thus, as-prepared biosensor offers a rapid, label-free and cost-effective approach for IgY quantification.
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