Related Experiment Video
Updated: Sep 23, 2025
Dynamic Monitoring of Seroconversion using a Multianalyte Immunobead Assay for Covid-19
Published on: February 16, 2022
Immunohistochemical Detection of SARS-CoV-2 Antigens by Single and Multiple Immunohistochemistry
Silvia Lonardi1, Mattia Bugatti2, Arianna Valzelli1
1Section of Pathology, Department of Molecular and Translational Medicine, University of Brescia, Spedali Civili di Brescia, Brescia, Italy.
Insights
Immunohistochemistry (IHC) detects SARS-CoV-2 in tissues. Multi-staining techniques enhance precision for identifying infected cells and analyzing tissue structures, improving diagnostic efficiency.
Area of Science:
- Pathology
- Immunology
- Virology
Background:
- Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection requires accurate detection in tissue samples.
- Immunohistochemistry (IHC) is a valuable tool for visualizing specific antigens within cells and tissues.
Purpose of the Study:
- To highlight the utility of immunohistochemistry (IHC) for detecting SARS-CoV-2 in tissue sections.
- To demonstrate the advantages of double or triple immunostaining and immunoenzymatic multi-staining for detailed cellular and tissue analysis.
Main Methods:
- Utilizing bright field microscopy with immunohistochemistry (IHC).
- Employing double or triple immunostains to identify infected cells and their relationships within tissue structures.
- Implementing immunoenzymatic multi-staining for simultaneous identification, localization, and enumeration of cellular epitopes.
Main Results:
- Immunohistochemistry (IHC) effectively localizes SARS-CoV-2 antigens in tissue sections.
- Multi-staining techniques accurately identify infected cells and their spatial relationships.
- Immunoenzymatic multi-staining allows for simultaneous analysis of multiple cellular targets.
Conclusions:
- Immunohistochemistry (IHC), particularly multi-staining methods, offers a precise and efficient approach for diagnosing SARS-CoV-2 infections in tissues.
- These techniques improve analytical precision and reduce quantification time, maximizing information from paraffin-embedded tissues.
Abstract:
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection can be demonstrated in tissue sections by immunohistochemistry (IHC), which has the power to localize in bright field specific antigens in cells and tissues. The use of double or triple immunostains is capable of highlighting which cells are infected and/or the relationship of infected cell with other cells and tissue structures. In addition, immunoenzymatic multi-staining permits the simultaneous identification, localization, and enumeration of different cellular epitopes. Moreover, this method improves analytical precision, decreasing the time required for morphometric quantification, maximizing the information obtained from a single slide of paraffin-embedded tissue.
More Related Videos
07:08A Rapid, Multiplex Dual Reporter IgG and IgM SARS-CoV-2 Neutralization Assay for a Multiplexed Bead-Based Flow Analysis System
Published on: April 6, 2021
08:07Author Spotlight: Advancing Antiviral Strategies Through Novel Immunocapture and Mass Spectrometry Techniques
Published on: January 12, 2024
