Diagnostic Value of Peripheral Blood Lymphocytes for Primary Immune Thrombocytopenia
Jun Lu1,2, Ling Sun1,2, Xifeng Wu1,2
1Jinan People's Hospital Affiliated to Shandong First Medical University, Jinan, China.
Insights
Absolute peripheral blood lymphocyte counts show high diagnostic value for primary immune thrombocytopenia. This method offers high sensitivity and specificity, aiding early detection and targeted treatment for patients.
Area of Science:
- Hematology
- Immunology
- Clinical Diagnostics
Background:
- Primary immune thrombocytopenia (ITP) is an autoimmune disorder characterized by low platelet counts.
- Accurate and early diagnosis of ITP is crucial for effective patient management and treatment.
- Assessing peripheral blood lymphocyte subsets may offer insights into ITP pathogenesis and diagnosis.
Purpose of the Study:
- To investigate the diagnostic value of absolute peripheral blood lymphocyte counts in patients with primary immune thrombocytopenia.
- To compare lymphocyte subset levels between ITP patients and healthy controls.
- To evaluate the sensitivity and specificity of absolute lymphocyte counts for ITP diagnosis.
Main Methods:
- A cohort study comparing 76 ITP patients with 80 healthy controls.
- Measurement of absolute peripheral blood lymphocyte counts and subsets (CD3+, CD3+CD4+, CD4+/CD8+, CD16+CD56+, CD3+CD8+, CD19+, ALC) using a fully automated hematology analyzer.
- Statistical analysis to compare values between groups and determine diagnostic accuracy.
Main Results:
- ITP patients exhibited significantly different levels of various lymphocyte subsets compared to controls (P < 0.05).
- Specific patterns were observed in newly diagnosed, relapsed/refractory, mild, and severe ITP cases.
- Absolute lymphocyte counts demonstrated high diagnostic accuracy with 93.42% sensitivity and 90.00% specificity for ITP.
Conclusions:
- Absolute peripheral blood lymphocyte counts are valuable diagnostic markers for primary immune thrombocytopenia.
- This method can significantly improve early diagnosis rates, enabling timely and targeted treatment.
- The findings support the clinical application of absolute lymphocyte counts for enhanced ITP detection and management.
Abstract:
The purpose of this study was to investigate the absolute value of peripheral blood lymphocytes in patients with primary immune thrombocytopenia and the diagnostic effect on patients with primary immune thrombocytopenia. From January 2020 to June 2021, 76 patients with primary immune thrombocytopenia and 80 healthy check-ups admitted to our hospital were selected as study subjects and divided into a control group (80 patients, healthy check-ups) and an observation group (76 patients, primary immune thrombocytopenia), according to the health status of the organism. Early morning fasting venous blood was collected from both groups, and the absolute value of peripheral blood lymphocytes was measured and compared using a fully automated hematology analyzer to investigate the diagnostic value of absolute peripheral blood lymphocytes in primary immune thrombocytopenia. The CD3+, CD3+CD4+, CD4+/CD8+, and CD16+CD56+ assay values in the observation group were lower than those in the control group, and the CD3+CD8+, CD19+, and ALC assay values were higher than those in the control group (P < 0.05). The CD3+CD8+ detection values of newly diagnosed patients were similar to those of relapsed refractory patients (P > 0.05); CD3+, CD3+CD4+, CD4+/CD8+, and CD16+CD56+ detection values of newly diagnosed patients were lower than those of relapsed refractory patients, and CD19+ and ALC detection values were higher than those of relapsed refractory patients; CD3+, CD3+CD4+, CD4+, CD4+/CD8+, and CD16+CD56+ detection values of mild patients were lower than those of relapsed refractory patients; CD3+, CD3+CD4+, CD4+/CD8+, and CD16+CD56+ detection values were higher in mild patients than in severe patients, and CD3+CD8+, CD19+, and ALC detection values were lower than in severe patients (P < 0.05). The absolute lymphocyte values were of high diagnostic value in primary immune thrombocytopenia, with a sensitivity and specificity of 93.42% and 90.00%. The application of absolute peripheral blood lymphocyte value in the clinical diagnosis of primary immune thrombocytopenia can achieve a better detection and diagnosis effect, which has a positive impact on the early diagnosis rate and can help patients to obtain more timely, effective and targeted treatment, and is worthy of promotion.
