Reference Ranges of Lymphocyte Subsets in Healthy Individuals from Southern Iran
Insights
This study establishes normal reference values for lymphocyte subsets in healthy individuals from southern Iran. These values are crucial for diagnosing and managing immune-related disorders in specific populations.
Area of Science:
- Immunology
- Hematology
Background:
- Lymphocyte immune-phenotyping is vital for diagnosing and monitoring various medical conditions.
- Establishing population-specific reference values is essential for accurate clinical interpretation.
Purpose of the Study:
- To determine the reference values of lymphocyte subsets in healthy individuals from southern Iran.
- To provide a basis for the clinical management of immune-related disorders.
Main Methods:
- Flow cytometry was employed to analyze peripheral blood samples.
- The study included 86 healthy subjects from southern Iran.
- Frequencies of T cells, B cells, NK cells, and their subsets were quantified.
Main Results:
- Mean percentages for lymphocyte subsets (T cells, B cells, T helper cells, T cytotoxic cells, activated T cells, NK cells, NKT cells) were established.
- The CD4+/CD8+ ratio was determined to be 1.22 ± 0.4.
- Helper T-cell percentages positively correlated with age, while cytotoxic T-cell and NK cell percentages negatively correlated with age.
Conclusions:
- The study provides normal reference ranges for peripheral blood lymphocyte subsets in a healthy Iranian population (ages 15-55).
- These findings underscore the importance of population-specific reference values for lymphocyte subsets.
- Establishing these ranges aids in the monitoring and clinical management of immune disorders.
Background:
Lymphocyte immune-phenotyping is considered as a useful tool in diagnosis and monitoring different medical conditions. This study aimed to establish a reference value of different lymphocyte subsets in healthy individuals from southern Iran.
Methods:
Flow cytometric method was used to determine the frequency of T cells, helper T cells, cytotoxic T cells, activated T cells, NK cells, NKT cells, and B cells in peripheral blood of 86 healthy subjects from southern Iran.
Results:
Regardless of gender, the mean percentage of the lymphocyte subsets have been observed as T cells (69.2 ± 8.84%), B cells (11.88 ± 4.14%), T helper cells (39.85 ± 7.75%), T cytotoxic cells (33.97 ± 6.64%), activated T cells (6.67 ± 3.60%), NK cells (12.56 ± 7.32%), and NKT cells (5.07 ± 2.83%). The ratio of CD4+/CD8+ was found to be 1.22 ± 0.4. An insignificant difference was found between lymphocytes of male and female. The percentage of helper T-cells has demonstrated a positive correlation and the percentage of cytotoxic T-cells and NK cells have il-lustrated a negative correlation with age.
Conclusions:
The results provide normal reference values of peripheral blood lymphocyte subsets in healthy individuals from southern Iran with the age range of 15 - 55 years old. Moreover, the results support this approach that reference values of lymphocyte subsets should be provided for each population. The reference range value of circulating lymphocytes is a powerful tool for monitoring and/or clinical management of immune related disorders.
