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Lymphocyte Transformation Test Based on Lymphocyte Changes Observed by a Hematology Analyzer before and after
Lulu Zhang1, Tinghua Feng1, Hui Liu1
1College of Medical Laboratory, Dalian Medical University, Dalian, China.
Insights
This study introduces an objective lymphocyte transformation test using a hematology analyzer for cellular immune function assessment. Patients with hematopathy showed significantly lower lymphocyte transformation rates compared to healthy individuals.
Area of Science:
- Immunology
- Hematology
- Clinical Diagnostics
Background:
- The classical lymphocyte transformation test (LTT) for cellular immune function relies on subjective interpretation.
- There is a need for objective and quantifiable methods to assess immune responses.
Purpose of the Study:
- To establish a novel, objective lymphocyte transformation test using a hematology analyzer.
- To evaluate the clinical utility of this objective LTT in distinguishing between patients with hematopathy and healthy individuals.
Main Methods:
- Whole blood cells were cultured and stimulated with phytohemagglutinin.
- Lymphocyte counts and transformation rates were determined using a hematology analyzer.
- Peripheral blood samples from patients with hematopathy and healthy controls were analyzed.
Main Results:
- A statistically significant difference was observed between stimulated and unstimulated blood groups (P < 0.05).
- Patients with hematopathy exhibited significantly lower peripheral blood lymphocyte transformation rates compared to healthy individuals.
Conclusions:
- Lymphocyte transformation can be reliably observed and quantified using a hematology analyzer.
- This objective LTT method holds significant clinical value for assessing cellular immune function, particularly in hematological conditions.
Objective:
The lymphocyte transformation test is a classical test for the detection of cellular immune function and is based on subjective judgment. In this study, we have established an objective novel lymphocyte transformation test using the hematology analyzer to observe lymphocyte transformation.
Methods:
Whole blood cells were cultured using a whole blood method with a lymphocyte culture medium; phytohemagglutinin was used to stimulate the experimental samples, and control was set up at the same time. After the whole blood cells were cultured, the number of lymphocytes in the two groups was observed using a hematology analyzer, and the conversion rate was calculated. The new method was used to observe differences in lymphocyte conversion in the peripheral blood of patients with hematopathy and healthy persons.
Results:
There were significant differences between the stimulated peripheral blood group and the blank group. The transformation rate of peripheral blood lymphocytes in patients with hematopathy was significantly lower than that in healthy persons; the difference was statistically significant (P < 0.05).
Conclusion:
Lymphocyte transformation can be observed using a hematology analyzer. The lymphocyte transformation test that is based on the determination of lymphocyte count by a hematology analyzer has important clinical value.
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